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The Fat-Soluble Vitamins

The Fat-Soluble Vitamins. Zhihong Li (李志红) Department of Biochemistry. Fat Soluble Vitamins. Fat soluble vitamins have long nonpolar hydrocarbon chains or rings . Common fat soluble vitamins include A, D, E, K. Vitamin D. Vitamin K. 2. Fat Soluble Vitamins.

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The Fat-Soluble Vitamins

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  1. The Fat-Soluble Vitamins Zhihong Li(李志红) Department of Biochemistry

  2. Fat Soluble Vitamins • Fat soluble vitamins have long nonpolar hydrocarbon chains or rings. • Common fat soluble vitamins include • A, • D, • E, • K. Vitamin D Vitamin K 2

  3. Fat Soluble Vitamins • Fat soluble vitamins usually accumulate in tissues and are not leached out quickly. • Unlike water soluble vitamins, an excess of a fat soluble vitamin can be just as harmful as a deficiency. 3

  4. 1. Vitamin A (Retinol) • Foods from animal source provide various retinoids that are easily converted to retinol in the body • Foods from plant source provide carotenoids • Over 500 carotenoids found in nature • Less than 10% with provitamin A activity • -carotene highest activity • Must be acted upon in the gut or by the liver to form retinol

  5. Vitamin A - Sources • Animal sources • Liver • Milk • Egg yolk • Plant sources • Carrots • Green leafy vegetables • Orange coloured fruits, e.g. mango and apricots

  6. RDA (retinol): • 1000 μg of retinol, or 3,500 IU, for males • 800 μg of retinol, or 2,500 IU, for females • one glass of milk provides 10% of the RDA • one medium carrot provides 200% of the RDA • a serving of liver provides 900% of RDA 6

  7. The Retinoids • 3 forms of vitamin A important for health • Retinol (key player; can be converted to other forms) • Retinal • Retinoic acid • β-carotene • potent provitamin A

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  9. Carotenoid Cleavage and Storage • β-carotene is converted to vitamin A in the intestinal mucosa • 90% is stored in liver, mainly as the ester, retinyl palmitate (~ 6 months storage) • Small amounts in adipose and blood • Transport • Retinol binding protein(RBP) acts to transport vitamin A from the liver and in the blood. • Excretion • Small amount in urine

  10. Major function in the body vital to good vision prevents night blindness antioxidant (β-carotene) necessary for healthy skin, hair growth keeps mucous membranes healthy promotes bone development Support immunity (retinoic acid and carotenoids) 10

  11. Role of vitamin A in vision

  12. Rhodopsin cycle or Wald’s Visual Cycle • Rhodopsin: combination of a protein part (opsin) and 11-cis retinal (only cis form can bind with opsin). • When light energy is absorbed by rhodopsin, the rhodopsin decompose: converts 11-cis retinal into all-trans retinal. • In order to maintain the ability to detect light, the rods must reconvert the all-trans retinal into 11-cis retinal. • This process only occurs under the dark environment. • Dark adaptation time

  13. Vitamin A - Deficiency • More obvious deficiency symptoms than other vitamins • Night blindness • Leading cause of blindness in third world countries • Cell keratinization • Dry skin • Xerophthalmia (dryness of cornea & conjunctiva) • Reproductive failure • Abnormal skeletal development/maintenance • Immune dysfunction

  14. Xerophthalmia curable early stage after 8 days vitamin A  incurable late stages

  15. Vitamin A Toxicity • Not readily excreted • Hypervitaminosis A in humans • Self-medication and overprescription • symptom • Skeletal malformations, spontaneous fractures • Birth defects and miscarriage • Decalcification, joint pain, fragility • Dry itchy skin • Hair loss • Liver damage • Overconsumption of beta carotene from food sources may cause skin to turn yellow but is not harmful

  16. Beneficial effects of β-carotene • Antioxidant role of β-carotene • Increased consumption of β-carotene is associated with decreased incidence of heart attacks, skin and lung cancers. • High doses of carotene for long periods are not toxic.

  17. 2. Vitamin D: Sunshine Vitamin • Body can make it if exposed to enough sunlight • Made from cholesterol in the skin

  18. Vitamin D - Sources • Synthesized in body • Plants (ergosterol) • Sun-cured forages • Fluid milk products are fortified with vitamin D • Oily fish • Egg yolk • Butter • Liver • Daily Value • 10 micrograms

  19. Vitamin D (calciferol) There are 2 major precursor forms: 7-dehydrocholesterol Ergosterol Vitamin D2 = ergocalciferol Completely synthetic form produced by the irradiation of the plant steroid ergosterol Vitamin D3 = cholecalciferol Produced photochemically by the action of sunlight or ultraviolet light from the precursor sterol 7-dehydrocholesterol

  20. Formation of Vitamin D • Skin (UV light) • 7-dehydro cholesterol  vitamin D3 • Liver • OH-group added • 25-hydroxy vitamin D3 • Storage form of vitamin (~3 months storage in liver) • Kidney • OH-group added by 1-hydroxylase • 1,25-dihydroxy vitamin D3 (or 1,25-dihydroxy cholecalciferol, 1,25-DHCC) • Active form of vitamin D, a “steroid hormone” • OH-group added by 24-hydroxylase • 24,25-dihydroxy vitamin D3 • Inactive form of vitamin D, ready for excretion

  21. Calcitriol (1,25-dihydroxy-cholecalciferol, 1,25 DHCC)

  22. Chemical name Abbreviation Generic name Vitamin D2 D2 Ergocalciferol Vitamin D3 D3 Cholecalciferol 25(OH)D Calciferol 3 25-hydroxy Vitamin D3 1,25-dihydroxy 1,25-(OH)2D3 Calcitriol vitamin D3 24,25-dihydroxy 24,25- (OH)2D3 Secalcifediol vitamin D3

  23. Functions of Vitamin D Calcium and Phosphorus Homeostasis Calcium and Phosphorus absorption (small intestine) Calcium resorption (bone and kidney) Maintain blood calcium levels Bone formation Stimulate calcium uptake for deposition as calcium phosphate (Osteoblasts: bone-forming cells) Hormone Regulation of gene expression Cell growth 24

  24. PTH:Parathyroid Hormone 25

  25. Vitamin D (antirachitic vitamin)- Deficiency • Less common • Young animals • Rickets (meaning: twist) • Failure of bones to grow properly • Results in “bowed” legs or knock-knees, outward bowed chest and knobs on ribs • Older animals • Osteomalacia(Greek: osteon-bone, malakia-softness): • Adult form of rickets • Osteoporosis (porous bones): • Loss of vitamin D activity with advancing age • Associated with fractures  very serious for geriatrics

  26. Osteoporosis • progressive loss of bone density, thinning of bone tissue and increased vulnerability to fractures in the elderly people of both sexes.

  27. Vitamin D Toxicity • Hypervitaminosis D • Among the vitamins, vitamin D is the most toxic in overdoses(10-100 times RDA) • Calcification of soft tissue • Lungs, heart, blood vessels • Hardening of arteries, stone formation in kidneys • Does not occur from sunlight or dietary sources • Does occur with supplementation • In general, vitamin D supplement are not recommended. • Upper limit: 50 micrograms a day

  28. 3. Vitamin E (tocopherol) • A naturally occurring antioxidant. • Essential for nomal reproduction in many animals. Tocopherol (Greek: tokos-child birth; pheros-bear; ol-alcohol), Anti-sterility vitamin.

  29. Vitamin E - Sources • Plant sources • Cereal grains • Especially in germ • Vegetable and seed oils • Animal sources • Meat,milk,butter,eggs • RDA: 10 mg for men, and 8 mg for women

  30. Vitamin E (tocopherol) • The term vitamin E refers to a family of 8 related compounds, the tocopherols and the tocotrienols • The four major forms of vitamin E are designated a, b, d, g, of which a-tocopherol is the most active.

  31. Absorption, Transport and Excretion of Vitamin E Absorption and Transportation Micelles into chylomicrons Transported via lipoproteins Stored in adipose tissue Excretion Bile, urine and skin 33

  32. Major function in the body Antioxidant Vitamin E is very unstable, easily oxidized protect cells against oxidative damage by free radicals, for example oxidation of the lipids in the cell membranes plays a role in aging, sexual performance, or preventionof cancer and/or heart disease 34

  33. Vitamin E - Deficiency • Rare • Erythrocyte hemolysis and hemolytic anemia • disruption of red blood cell membranes, perhaps due to polyunsaturated fatty acids (PUFAs) oxidation • Sterility: reproductive failure

  34. Vitamin E Toxicity • Toxicity rare:wide range of safe intake compared to other fat soluble vitamins • Vitamin E is one of the most popular nutrient supplements. There are many health claims for supplementation – slowing of the aging process or an improvement in sexual potency.

  35. 4. Vitamin K • The only fat soluble vitamin with a specific coenzyme function. • Required for the production of blood clotting factors, essential for coagulation. Named asantihemorrhagic vitamin.

  36. Vitamin K - Sources • Plant sources • Green leafy vegetables • Some oils • Broccoli • Animal sources • Liver • Milk • Also made by bacteria in the gut • RDA: 80 µg for men, 65 µg for women

  37. Forms of Vitamin K • K1, phylloquinone • Chloroplasts in plants • K2, menaquinone • Bacterial synthesis • K3, menadione • Synthetic, Complexed to improve stability Vitamin K2

  38. Absorption, Transport and Excretion of Vitamin K Absorption and Transportation Absorbed in small intestine, via chylomicrons in lymphatic system. Transported via lipoproteins and stored in the liver Excretion Primarily bile, small amount urine 41

  39. Functions of Vitamin K • Essential for clotting of blood. • Clotting factors are synthesized in the liver as inactive precursors - vitamin K converts them to their active forms • Conversion of prothrombin to thrombin, an active enzyme • Formation of fibrinogen to fibrin, leading to clot formation • Stimulates bone formation and decreases bone resorption.

  40. Deficiency: increased bleeding and hemorrhage rare, they may occur in some individuals when antibiotic medications kill the intestinal bacteria that produce the vitamin. Hypervitaminosis K: thrombosis, hemolytic anaemia and jaundice, particularly in infants. The toxic effect is due to increased breakdown of RBC. 44

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