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POLIMERASE CHAIN REACTION

POLIMERASE CHAIN REACTION. Juanita Salgado Nicolás Borrero Nicolás Jaramillo. P.C.R. It is a technique used to produce large quantities of replicated DNA.

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POLIMERASE CHAIN REACTION

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  1. POLIMERASE CHAIN REACTION Juanita Salgado Nicolás Borrero Nicolás Jaramillo.

  2. P.C.R • It is a technique used to produce large quantities of replicated DNA.

  3. One of its uses is when having to analyze something of which you don’t have a lot of sample because by having larger quantities, the analysis is easier. • Examples: blood, semen, hair, saliva and other tissues (amplified using PCR).

  4. The PCR is required at high temperatures and also needs the help of the polymerase enzyme, found in Thermusaquaticus(bacteria).

  5. PROCESS • The DNA fragment of interest is taken away along with some nucleotides, primers and the enzyme. • They are placed at temperatures around 95°C so that it can be denatured (breaking hydrogen bonds that hold together the structure). Primer: a strand of nucleic acid that serves as a starting point for DNA synthesis.

  6. The temperature is reduced to 60°C so primers can form hydrogen bonds with the complementary base pairs of the DNA (following base pair rules).

  7. The temperature is raised to 72°C, so the enzyme works efficiently and begins polymerizing until both strands of the DNA are created. • This process repeats various times, having as a result, many copies of the same DNA fragment.

  8. Video

  9. Bibliography • http://www.sumanasinc.com/webcontent/animations/content/pcr.html • http://www2.le.ac.uk/departments/emfpu/genetics/explained/images/PCR-process.gif/image • http://openwetware.org/images/f/f5/CH391L_S12_PCR_process.gif

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