1 / 9

Standard BioBrick plasmid backbone

Goal: To persuade a bacterium to produce a variety of small peptides and secrete them into the medium. Peptide secretor cassette. X. E. P. S. antibiotic resistance. origin. Standard BioBrick plasmid backbone. (http://partsregistry.org/Plasmids). His. M. Cassette Structure.

finn
Download Presentation

Standard BioBrick plasmid backbone

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. Goal: To persuade a bacterium to produce a variety of small peptides and secrete them into the medium

  2. Peptide secretor cassette X E P S antibiotic resistance origin Standard BioBrickplasmid backbone (http://partsregistry.org/Plasmids)

  3. His M Cassette Structure Secretory signal GFP Peptide RBS

  4. His M Standard Promoter • e.g. J23100 – Strong, constitutive promoter Secretory signal GFP Peptide RBS

  5. His M Standard RBS • e. g. B0034 – star 1 and W Secretory signal GFP Peptide RBS

  6. His M Synthesize Secretory Signal • e. g. lipA or sacB – identified from registry • Possibly need to synthesize • Leave as an exercise for the student! Secretory signal GFP Peptide RBS

  7. His M Some random gene • Need a signaller… • … and a handle • Allow student contributions for alternatives Secretory signal GFP Peptide RBS

  8. His M The ‘slot’ in the cassette • Need: • 1 cleavage site • 1 target peptide • 1 purification handle Secretory signal Peptide GFP RBS

  9. His M What goes in the player? • The polymer engineer is thinking about, e.g. extracellular matrix proto-molecules and self-assembling elements • The biologist is thinking about using this as a recruiting tool… Secretory signal Peptide GFP RBS

More Related