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The Project

Analysis of Androstenone , Skatole and Indole in Biopsy Boar Samples Ampuero Kragten 1 , S., Robatel 1 , A., Mattei 2 , S., Bee 1 , G., Hofer 3 , A. 1 Agroscope Liebefeld - Posieux ALP, 2 University of Zurich, 3 SUISAG. The Project.

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The Project

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  1. Analysis of Androstenone, Skatole and Indole in Biopsy Boar SamplesAmpuero Kragten1, S., Robatel1, A., Mattei2, S., Bee1, G., Hofer3, A. 1AgroscopeLiebefeld-Posieux ALP, 2 University of Zurich, 3 SUISAG

  2. The Project • Developed at ALP as part of a breeding project from Suisag, Switzerland • Funded by the Swiss Commission of Innovation and Technology • Boars of Swiss Large White breed • Samples taken at the neck region Boarsheadingfor 2018, Amsterdam, 2. December 2011

  3. Biopsy / Liquid Fat Heavyboars Light boars R2 = 0.014 N = 6 R2 = 0.317 N = 14 Boarsheadingfor 2018, Amsterdam, 2. December 2011

  4. Biopsy / Liquid Fat Boarsheadingfor 2018, Amsterdam, 2. December 2011

  5. Biopsy / Liquid Fat Optimum microwaveheating cycle: 9min ( 3x3min; 600 W) Biopsy mass set to 300 mg Boarsheadingfor 2018, Amsterdam, 2. December 2011

  6. Biopsy / Liquid Fat 583 samples 58 replicates 2 samples ~0 LF 5 samples < 20 mg LF 42 samples < 40 mg LF Life Weight, mean (STD): 138 (61) kg Liquid Fat, mean (STD): 91.3 (40.1) mg Boarsheadingfor 2018, Amsterdam, 2. December 2011

  7. Extraction procedure 200 ml MeOH:H2O (95:5) + Stdint (androstanone + 2-methyl indole) (150 ml) 70 – 100 mgLiquid Fat (40 – 70 mg LF) Boarsheadingfor 2018, Amsterdam, 2. December 2011

  8. HPLC procedure Derivatization in-situ: 3 ml + 2 ml + 2 ml Dansylhydrazine+ H2O + BF3 + 20 ml extract 2 min Boarsheadingfor 2018, Amsterdam, 2. December 2011

  9. HPLC procedure Injection: 10 ml AndrostenoneSkatol, Indole Derivat.: yes no Column: SB C18 Eclipse XDB C18 Eluent: THF/phosphate buffer (50:50) MeOH/phosphate buffer (50:50) Flow rate: 1.3 ml/min 1.3 ml/min pH: 6 6 Temp.: 40°C 40°C FLD: 346/521 nm 285/340 nm Boarsheadingfor 2018, Amsterdam, 2. December 2011

  10. Biopsy vs. Normal Analysis Boarsheadingfor 2018, Amsterdam, 2. December 2011

  11. Biopsy vs. Normal Analysis Boarsheadingfor 2018, Amsterdam, 2. December 2011

  12. Conclusions • Biopsy method is adapted to breeding programs • A good compromise between biopsy size and critical analytical mass was found (0.9 % n.d.) • Analysis of skatole and indole is less problematic • Deviation in accuracy: <3% at 2.0 mg/kg A (70-100 mg LF) • Deviation in accuracy: <12% at 0.3 mg/kg A • or for analysis with < 70 mg LF • Good correlation with normal method Boarsheadingfor 2018, Amsterdam, 2. December 2011

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