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Staining HAEMATOXYLIN & EOSIN. Supervised by : Dr.Sherifa Prepared by: Reem Aldossari Lama Alabdi. H&E staining:

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Staining haematoxylin eosin

StainingHAEMATOXYLIN & EOSIN

Supervised by : Dr.Sherifa

Prepared by: Reem Aldossari

Lama Alabdi


Staining haematoxylin eosin

  • H&E staining:

  • The best known and most widely used staining procedure, hematoxylin-eosin staining (H&E), uses hematoxylin solutions for nuclear staining and eosin solutions for cytoplasmic staining.

  • In the first step, the nuclei are stained with a hematoxylin solution. The nuclei stain blue, dark violet to black.

  • The second step is counterstaining with eosin Y or eosin B.

  • In this process cytoplasm, collagen, keratin stain red.


Staining haematoxylin eosin

  • H&E staining is the standard staining method used in histology. It gives an overview of the structure of the tissue, enabling differentiation of the structures being examined as normal, inflammed,degenerative and pathological changes .

  • It is often possible to make a diagnosis based on the results of H&E staining, or it can be determined which special staining techniques are still necessary for subtyping.


Staining haematoxylin eosin

  • APPLICATION histology. It gives an overview of the structure of the tissue, enabling differentiation of the structures being examined as normal,

  • Gives a general overview of the tissue structure.

  • Can be used as a diagnostic tool.


Staining haematoxylin eosin

  • Advantages histology. It gives an overview of the structure of the tissue, enabling differentiation of the structures being examined as normal, of H&E staining

  • Combination of the two stains gives outstanding

  • results.

  • Ready to use reagents .

  • Easy to use.

  • Results in just two steps.


Staining haematoxylin eosin

  • Procedure for H & E Staining of Paraffin Sections: histology. It gives an overview of the structure of the tissue, enabling differentiation of the structures being examined as normal,

  • 1-(Fixative: Any general fixative is adequate. Ex.formalin)

  • 2--Deparaffinization in :

  • 3 - labeledXylene I ( 1-3 minutes)

  • 4- EtOH (ethanol alcohol) 100% (2-3 minutes

  • 5-EtOH(ethanol alcohol) 96% (2-3 minutes)

  • 6-EtOH(ethanol alcohol) 80% (2-3 minutes)

  • 7-EtOH(ethanol alcohol) 70% (2-3 minutes)

  • 8-Dist. water, 2 minutes.

  • 9. Hematoxylin, approximately 10 minutes.

10-EtOH(ethanol alcohol) 70% (2-3 minutes)

11-EtOH(ethanol alcohol) 80% (2-3 minutes)

13-Eosin Y solution, 2 to 5 minutes.

16- Absolute 100% ethanol, 1-2 minutes.

18-Xylene I, 1-2 minutes.

21-Mounting with DPX

  • 12-EtOH(ethanol alcohol) 96% (2-3 minutes)


Staining haematoxylin eosin

Thank you histology. It gives an overview of the structure of the tissue, enabling differentiation of the structures being examined as normal,