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IB Solubilization

Paul Lem Stephanie Fisher Sarah Fleming. IB Solubilization. What is IB Solubilization ? Solubilization agents Reaction parameters and reactor design Economics. Outline. After Primary Recovery Section. Where in process is IB solubilization.

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IB Solubilization

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  1. Paul Lem Stephanie Fisher Sarah Fleming IB Solubilization

  2. What is IB Solubilization? Solubilization agents Reaction parameters and reactor design Economics Outline

  3. After Primary Recovery Section Where in process is IB solubilization

  4. Mis-folded proteins that aggregate together • By hydrophobic and intermolecular interactions • To a great extent, biologically inactive • Reversible process • A way the cell can cope with thermal stresses What is a inclusion body?

  5. Urea • Denaturant/Chaotropic agent • Prevents or interferes with intramolecular bonds • Van derwaal forces, hydrogen bonds, hydrophobicity in the secondary protein structure • 2-mercaptoethanol • Reducing Agent • Used in low concentration • Breaks di-sulfide bonds Solubilizing agents

  6. Batch reaction • Concentrations: • 300g/L (5 M) urea • 40g/L 2-mercaptoethanol • Temp 35OC • pH above 9 • Batch size of 10,184 L • 90% Conversion • 8 hours reaction time Reaction Parameters

  7. Glass-Lined Stainless Steel Reactor Calculated from Superpro and than scaled up to be 85% of the reactor size 3000 gallon reactor Reactor Design

  8. De Dietrich Gl-3000 • $170 000

  9. Ra-96 Series DS81-100d-2 $185 000

  10. Rs-96 Series Ds81-500d-1 $200 000

  11. Urea • $466.05/12 kg • Ultrapure • 3120 kg/batch • 2-Mercaptoethanol • $59/500 g • Ultrapure • 416 kg/batch • De Dietrich GL-3000 Reactor • $170 000 • 9.08 kg/hr steam required Economics

  12. Questions?

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