1 / 28

Aphid-Borne Barley Yellow Dwarf Luteo Virus (BYDV)

Aphid-Borne Barley Yellow Dwarf Luteo Virus (BYDV). Viruses identified on Egyptian wheat and barley. Leafhopper-borne wheat dwarf mono- geminivirus (WDV) 2. Seed-borne barley stripe mosaic hordeivirus (BSMV) 3. Leafhopper-borne maize yellow stripe tenuivirus -like (MYSV)

fauna
Download Presentation

Aphid-Borne Barley Yellow Dwarf Luteo Virus (BYDV)

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. Aphid-Borne Barley Yellow Dwarf Luteo Virus (BYDV)

  2. Viruses identified on Egyptian wheat and barley • Leafhopper-borne wheat dwarf mono- • geminivirus (WDV) • 2. Seed-borne barley stripe mosaic hordeivirus (BSMV) • 3. Leafhopper-borne maize yellow stripe tenuivirus-like (MYSV) • 4. Aphid-borne barley yellow dwarf luteovirus (BYDV).

  3. History

  4. History • 1982,Egyptian National Campaign • For wheat improvement has started • 1984,BYDV infection has been • recorded as sporadic single- infected plants in wheat fields of Lower Egypt (Abdel-Hak, 1984) • 1991,BYDV-RMV possible causal of severe outbreak in Mid Egypt maize fields (Aboul-Ata, et al, 1996)

  5. 1992,BYDV-PAV has been recorded and identified using Koch postulates (Aboul-Ata, et al, 1992) 1994,First record of 5 BYDV- serotypes (PAV, MAV, RPV, RMV and SGV) occurrence using DAS- ELISA at Purdue Univ., Indiana, USA (Lister, et al, 1994).

  6. 1994-2003, Egypt-ICARDA cooperation through Nile Valley Project funded by European countries. It deals with cereal viruses affecting wheat and barley 1994-1999, Different Wheat and barley germplasm were received from CIMMYT every year for BYDV-infection evaluation. Breeding program against BYDV has started 1994 1998,BYDV-PAV typing was done at INRA, France (Mastari, et al, 1998)

  7. PROBLEM IDENTIFICATION

  8. b a • External symptoms • caused by BYDV • Wheat, b. Patch of • infection on wheat • c. Patch of infection on barley c

  9. 1. • Morphology of major aphid vectors of BYDV • 2. Virus particles of different BYDVs 2.

  10. 1. Barley yellow dwarf luteovirus (BYDV) has different serotypes i.e. MAV, PAV and SGV • 2. Cereal yellow dwarf polerovirus • (CYDV) has different serotypes i.e. RPV and RMV • 3. There are almost 25 aphid species vector of both CYDV and BYDV

  11. Sequences of the virus specific primers used in the multiplex RT-PCR detection method.

  12. RT-PCR amplified products. M: 100 bp DNA ladder; L1: WSMV; L2: BYDV-PAV; L3: BYDV-MAV; L4: BSMV: L5: BYDV-MAV and BYDV-PAV.

  13. 4. BYDVs are transmitted persistently by aphid species and 24-48 hr is enough period as AAP 5. Monogenic incomplete dominant Yd2 and Bdv1 genes are BYDV resistance. 6. Resistance-breaking strains are mutually occurred

  14. Cereal crop hosts of BYDV-PAV and RMV. Wild cereal plants as sources of BYDV-PAV infection are listed

  15. BYDV-PAV Etiology, Typing and Sub-Typing

  16. 1. BYDV-PAV was isolated using R. padi. Virus detection was done using TBIA (Aboul-Ata, et al, 1992). 2. BYDV has five serotypes: PAV, MAV, RMV, RPV and SGV. All of them were recorded in Egyptian cereals (Lister, et al, 1996) 3. Symptom severity range (very severe – very mild/serotype/crop)

  17. 4. Dominant BYDV-PAV serotype has 2 subtype i.e. cpA and cpB. using Serological analysis by MAbs, RFLP and SSCP were used for differentiation .

  18. BYDV-PAV sub-serotype (cpA and cpB) variants Healthy Control = Fresh weight (1.7) EW1 = Egypt isolate 1, EW2 = Egypt isolate 2

  19. Phylogenic tree of BYDV-PAV for different cpA and cpB Sub-serotypes

  20. BYDV Epidemiology And Non-insect Transmission

  21. Suction Trap for aphid insect sampling

  22. Monitoring of BYDV-vectored aphids in the season period using DAS-ELISA

  23. Liner relation between BYDV-vectored aphids and BYDV infection

  24. Non-insect inoculation Inoculation application for sauced seeds of wheat and maize kernels. One pin is used for virus inoculation into wheat seeds. More pines are used for virus inoculation into maize seeds

  25. Wheat breeding for BYDV resistance

  26. Yield losses caused by BYDV on commercial wheat varieties

  27. Insect inoculation for BYDV resistance • Mass rearing of • Healthy aphid (R. padi) • 2. Allow aphids to • acquire PAV-BYDV • 3. Inoculation of 3-leaf • stage wheat lines using • BYDV-vectored aphids. • Genotypic response against virus • 4. Spray insecticide to kill all insects used for Inoculation. • 5. Collecting field data and virus detection 45 days after inoculation.

  28. THANK YOU ALL SO MUCH

More Related