Cell segmentation. Oleg Sklyar, Gregoire Pau, EMBL-EBI Cambridge email@example.com. Experimental setup. RNAi cell-array End-point assay HeLa cells Channels Actin (TRITC) Tubulin (Alexa 488) DNA (Hoechst). Cell segmentation. Challenging problem Cells superposition Noisy channels
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Oleg Sklyar, Gregoire Pau, EMBL-EBI Cambridge
t too large: mangled nuclei
t too small: unseparated nuclei
(H - Hm) > t'
Step 1: Cmask Nmask
Step 2: Cmask Nmask Cbound
Actin Tubulin Actin+Tubulin Cbound