correcting sickle cell anemia n.
Download
Skip this Video
Loading SlideShow in 5 Seconds..
Correcting Sickle-Cell Anemia PowerPoint Presentation
Download Presentation
Correcting Sickle-Cell Anemia

Loading in 2 Seconds...

play fullscreen
1 / 12

Correcting Sickle-Cell Anemia - PowerPoint PPT Presentation


  • 116 Views
  • Uploaded on

Correcting Sickle-Cell Anemia . Group B4 Jessica Hambrick Yichen Wang Tyler Fernandez Shawn Haley. Introduction. Hemoglobin : Oxygen-carrying protein Makes up Mammalian red blood cells Tetramer: contains 4 polypeptides (Freeman 2011). http://www.wikipedia.org/. (Freeman 2011).

loader
I am the owner, or an agent authorized to act on behalf of the owner, of the copyrighted work described.
capcha
Download Presentation

PowerPoint Slideshow about 'Correcting Sickle-Cell Anemia' - euclid


An Image/Link below is provided (as is) to download presentation

Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author.While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server.


- - - - - - - - - - - - - - - - - - - - - - - - - - E N D - - - - - - - - - - - - - - - - - - - - - - - - - -
Presentation Transcript
correcting sickle cell anemia

Correcting Sickle-Cell Anemia

Group B4

Jessica Hambrick

Yichen Wang

Tyler Fernandez

Shawn Haley

introduction
Introduction
  • Hemoglobin: Oxygen-carrying protein
  • Makes up Mammalian red blood cells
  • Tetramer: contains 4 polypeptides

(Freeman 2011)

http://www.wikipedia.org/

(Freeman 2011)

slide3

Sickle-cell anemia

  • Autosomal recessive trait
  • Primary structure of hemoglobin differs
  • If oxygen concentrations in the blood low, hemoglobin CRYSTALLIZES
  • Causes sickle-shape
  • Cells stuck in capillaries, block flow downstream
  • Cells starved for oxygen

(Freeman 2011)

http://familydoctor.org

slide4

Transcription of Genes

  • Transcription: RNA is made from DNA template
  • Transcription factor: protein that regulates transcription
  • Regulation of gene transcription: means by which gene expression is controlled to produce different proteins

(Latchman 2011)

data results
Data/Results
  • Fetal hemoglobin (HbF) vs. adult hemoglobin (HbA)
  • Tested mice with human genes affected with Sickle-cell anemia
  • Fetal hemoglobin in adults: lessons severity of symptoms

(Xuet al. 2011)

data results1
Data/Results

BCL11A

  • A “transcriptional repressor”
  • Involved in “switching” of fetal hemoglobin to adult hemoglobin
  • Silences fetal hemoglobin in adult cells
  • The experiment involved inactivating it

(Xuet al. 2011)

data results2
Data/ Results
  • In mice genetically engineering with human Sickle-cell hemoglobin…
  • Inactivated BCL11A
  • Hemoglobin switching failed in fetal liver
  • Lots of fetal hemoglobin, but levels declined after birth

(Xuet al. 2011)

data results3
Data/Results
  • Can fetal hemoglobin be reactivated?
  • YES!
  • Silencing of fetal hemoglobin reversible
  • Loss of BCL11A enhances effects in fetal hemoglobin inducers
  • Sickle-cell phenotypes corrected in adults

(Xuet al. 2011)

discussion
Discussion
  • Inactivation of a transcription factor can prevent expression of a gene:
    • Transcription factor = BCL11A
    • Gene expressed= fetal hemoglobin
  • HUGE STEP in finding therapy for hemoglobin disorders such as Sickle-Cell

(Xuet al. 2011)

conclusion
Conclusion
  • Sickle-cell anemia can be treated by the inactivation of a transcription factor!
literature cited
Literature Cited

Freeman, Scott. Biological Science. 4th ed. San Francisco, CA: Benjamin Cummings, 2011. Print.

Latchman, D. S. “Transcriptional Gene Regulation in Eukaryotes.” Nov. 2011. eLS. Web. 11 Nov. 2012.

Xu, Jian, et al. “Correction of Sickle Cell Disease in Adult Mice by Interference with Fetal Hemoglobin Silencing.” Science 334 (2011): 993-996. Medline.Web. 11 Nov. 2012.