Introduction to Genetics

1. Introduction to Genetics Simple Mendelian Traits and Monohybrid Punnett Squares

2. Traits Inventory

3. What is genetics? • Genetics is the scientific study of heredity or how traits (such as hair color, eye color, height, and even genetic disorders) pass from parent to offspring

4. Discovering Genetics • In the mid-1800s, an Austrian monk named Gregor Mendel studied how traits were passed from one generation to the next using pea plants • He noticed that some traits (like height or flower color) appeared in predictable patterns in the next generations. • His ideas form the foundation of the principles of heredity, known as Mendelian Genetics

5. Homologous Pairs and Genetics • Genetics depends on the homologous pairs we discussed in Meiosis. Each parent gives one chromosome from each pair to the offspring. Sometimes these pairs are exactly the same, and sometimes they are different versions (Ex - XX vs XY). The offspring then uses their new pair (one from mom and one from dad) to determine their own traits. • Identify the following as HOMOZYGOUS or HETEROZYGOUS • AA • Aa • aa

6. Studying Genetics • In order to study genetics, Mendel created purebreds through self-pollination. This guaranteed that the parent only had one type of trait to pass on (Homologous pairs were the same. Example BOTH purple or BOTH white) • After the purebreds were established, Mendel crossbred to create hybrids or individuals that contain a mix of DNA (homologous pairs that are different. Example - one purple and one white) • Identify the following as PUREBRED or HYBRID… • AA - homozygous dominant • Aa - heterozygous • aa - homozygous recessive

7. Tracking Traits • Mendel studied 7different traits (specific characteristics), such as seed color or plant height • He tracked the traits through several generations and recorded what trait was seen in the offspring. • The parent generation was called the P generation • The offspring from those parents are called the F1generation (first filial) • The offspring of the F1 were called the F2 etc.

8. The Results

9. Mendel’s Conclusions • Biological inheritance is determined by factors (genes) that are passed from one generation to the next. Each trait is controlled by one gene occurring in two contrasting forms – the different forms of each gene are called alleles (Example, the gene for plant height has a tall allele and a short allele.)

10. Mendel’s Conclusions 2. Law of Dominance- Some alleles are dominant and others are recessive. This can be determined by which allele shows in a hybrid individual. Example – a cross between a purebred tall parent and a purebred short parent will give 100% tall offspring. Therefore, the tall allele is dominant and the short allele is recessive. Recessive alleles will be shown ONLY if no dominant allele is present.

11. What happened to the recessive allele? • Mendel then crossed the F1 generation (hybrid with hybrid) and noted that the recessive trait reappeared about 25% of the time in the F2 generation. The recessive trait still transferred to the F1 generation, but it was hidden by the dominant trait.

12. Law of Segregation • Alleles (found in homologous pairs) are segregated (separated) from each other during meiosis so that each gamete (egg, sperm) carries only a single copy of each gene • New pairs form during fertilization

13. Genetics is NOT guaranteed! • We never know which sperm and which egg will be used to create the offspring, so genetics is entirely based on probability (the likelihood that an event will occur.) • For example, coin flipping: • the probability of flipping heads is ½ (50%) • the probability of flipping three heads in a row is ½ x ½ x ½ = 1/8 (1 in 8 chance) • For example, gender determination (X or Y sperm?) • The probability of getting a boy is ½ (50%) • The probability of getting a boy three times in a row is ½ x ½ x ½ = 1/8 (1 in 8 chance) • Past outcomes do not affect future ones!

14. Punnett Squares • To determine the likelihood of a specific trait being passed on, a Punnett square is used. This is a diagram to identify all possible combinations of alleles in offspring when two parents are “crossed”.

15. Two types of alleles • In Punnett Squares, DOMINANT alleles are represented by CAPITALletters ex:T is for tall • recessivealleles are represented by the same letter, but in lowercaseform ex:t is for short

16. Dominant or recessive? • Which of the following alleles are DOMINANT? A t q R s • Which of the following alleles are DOMINANT? A t q R s • Which of the following alleles are recessive? A t q R s • Which of the following alleles are recessive? A tq R s

17. Genotypes • Each person has 2 alleles in each pair – one from mom, one from dad; called the genotype • homozygous means you have 2 of the same allele (either both dominant, TT, or both recessive, tt) also known as purebred • heterozygous means you have 2 different alleles (one dominant, one recessive: Tt) also known as hybrid

18. Homozygous or heterozygous? • Label the following as homozygous (homoz) or heterozygous (heteroz): Hh heteroz BB homoz rr homoz Ss heteroz Tt heteroz qq homoz HH homoz • Label the following as homozygous (homoz) or heterozygous (heteroz): Hh BB rr Ss Tt qq HH

19. Genotype and phenotype • A Punnett square can be used to predict the genotypes and phenotypes possible in the offspring • A genotype is the genetic make-up (the actual alleles) ex: TT, tt, or Tt • A phenotype is the physical characteristic (what it looks like) ex: tall or short

20. Monohybrid Crosses • Although you inherit a lot of genes from your parents, monohybrid Punnett squares only look at a single gene or one trait at a time

21. Monohybrid Crosses • Place parents’ genotypes along the top and side. These must be determined through inferences. For example, a parent showing a recessive trait MUST be homozygous recessive. • Fill in the square to find the possible offspring genotypes.

22. Monohybrid Crosses • Try It: cross a homozygous tall plant with a homozygous short plant