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Sedimentation (24.7). When macromolecules move in solution, their motion is dictated by the forces they feel Gravitational force (F g ) is opposed by frictional (F fr ) and buoyant (F b ) forces

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sedimentation 24 7
Sedimentation (24.7)
  • When macromolecules move in solution, their motion is dictated by the forces they feel
    • Gravitational force (Fg) is opposed by frictional (Ffr) and buoyant (Fb) forces
    • Specific volume of the solute is the change in solution volume upon addition of a given amount of solvent (measure in cm3/g)
  • When all forces sum to zero, terminal velocity is achieved (vter)
  • Sedimentation coefficient relates speed of sedimentation to properties of macromolecule and solvent
    • Measured in units of Svedbergs (S) where 1S = 1 x 10-13 s
velocity sedimentation 24 7
Velocity Sedimentation (24.7)
  • Velocity sedimentation utilizes centripetal force instead of gravity
    • ω is the angular velocity (i.e., speed of centrifuge) and x is the distance between the particle and the center of rotation
  • Centrifugation causes heavier particles (solute) to move away from center of rotation
    • Boundary forms with one side being solution and the other side solvent
    • Sedimentation coefficient can be determined by monitoring motion of boundary
  • Sedimentation coefficients can give us molar mass (M = mNA) of the macromolecule if we know the frictional coefficient
    • We can get this from a diffusion experiment