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Mitotic spindle

Kinesin-5 nano-motors in cell division Leah Gheber Departments of Clinical Biochemistry and Chemistry Ilse Katz Institute for Nanoscale Science and Technology Ben-Gurion University of the Negev, Beersheba, Israel. human cells – specific kinesin-5 inhibitor monastrol. 5  m. control.

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Mitotic spindle

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  1. Kinesin-5 nano-motors in cell division Leah GheberDepartments of Clinical Biochemistry and ChemistryIlse Katz Institute for Nanoscale Science and Technology Ben-Gurion University of the Negev, Beersheba, Israel

  2. human cells – specific kinesin-5 inhibitor monastrol 5 m control monastrol Leizerman et al., CMLS 2005 (Gheber lab) Mitotic spindle

  3. Kinesin-5 + - - + Kinesin-5 motor proteins Spindle assembly Maintenance of bipolar spindle structure Anaphase spindle elongation

  4. Our Goal To understand the mechanisms and regulation of the mitotic kinesin-5 nano motors Our Strategy To correlate between in vivo functions and in vitro motor activity of specific kinesin-5 mutants and variants

  5. Cin8 Cin8 cin8-5A Cin8-3A 26 37 26 37 In vivo assays – protein expression and quantitative live imaging Cin8-3GFP Cin8-5A-3GFP Microtubule plus-end tracking Tubulin Kinesin-5

  6. In vitro - Single molecule fluorescence motility assay - Kinesin-5-3GFP

  7. Vladimir Natalia Nurit Adina Alina Rachel Ofer People who do the work Vladimir Fridman Adina Gerson-Gurwitz Natalia Movshovich Maria Podolskaya Rachel Abunie-Masala Ofer Shapira Nurit Schkolnik Alina Goldstein Collaborators Christoph Schmidt – GAUG Stefan Lakamper – GAUG Mart Loog – Tartu, Estonia Liam Holt – UC Berkeley, US Andy Hoyt – JHU, US Dina Raveh – BGU, Israel

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