General Microbiology. Group 1 Chimi Seldon Dorji Sangay Wangmo Tek Bahadur Powdel Sang Dorji Tamang Hem Raj Subba Norbu ( 14/04/2010). Definition Study of living organism of microscopic size
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Chimi Seldon Dorji
Tek Bahadur Powdel
Sang Dorji Tamang
Hem Raj Subba
Norbu ( 14/04/2010)
Eg.:Bacteria, fungi, algae, protozoa, and the infectious agents called viruses.
Example: Some micro-organisms are involved in making of yogurt, cheese and wine, in production of penicillin, interferon and alcohol, processing of domestic and industrial wastes
The walls of gram positive bacteria have more peptidoglycans (the large molecular network of repeating disaccharides attached to chains of four or five amino acids) than do gram-negative bacteria. Thus, gram-positive bacteria retain the original violet dye and cannot be counterstained.
Mix the agar according to the manufacturer's instructions. Agar is a bit like Jell-o and the ingredients will need to be heated to precise temperatures. Make certain that you have a clean cooking thermometer handy.
Open a petri dish and pour just enough agar into the dish to cover the bottom completely. Put the lid back on immediately and set the dish aside until the agar firms. Fill each dish the same way, never leaving the top of the dish off any longer than absolutely necessary. Once the agar hardens you may store the dishes in the refrigerator upside down to avoid the possibility of any airborne bacteria getting into the dishes.
Remove the dish from the cupboard. There is no need to open the dish. Observe the bacteria colonies through the top of the dish. Record their color, shape and anything else distinguishing. Look in a bacteria book or on the Internet for illustrations that match the bacteria you have cultured.
Destroy your cultured bacteria once you have identified it. Pour 1 tbsp. of bleach into the petri dish and then seal the dish closed with packing tape, put the dish in a baggie, seal it and throw away.
Causative agents of disease; diagnostic procedures; diagnostic procedures for identification of causative agents, preventive measures
Water purification, microbiological examination, biological degradation of waste,ecology
Contamination and soilage; dissemination of diseases
Food preservation and preparation; foodborne diseases and their prevention
Soil fertility, plant and animal diseases
Producton of medicinal products such as antibodies and vaccines; fermented beverages; industrial chemicals, production of proteins and hormones by genetically engineered microorganisms.
Exploration for life in the outer space
Coal, mineral and gas formation; prospecting for deposits of coal, oil, and gas; recovery of minerals from low-grade ores