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Actual Animal Genetic Resources in Slovakia. P. Chrenek, Makarevich A., Bulla J. firstname.lastname@example.org DAGENE 2016. Introduction.
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Actual Animal Genetic Resources in Slovakia P. Chrenek, Makarevich A., Bulla J. email@example.com DAGENE 2016
Introduction Ratification of the Convention on the Biodiversity wasconfirmed by SlovakRepublicalso in 1994, to protect biodiversity, to guarantee sustainable use of its components includealso animal genetic resources. Generally, the importance of animalgeneticresourcesprotection emerge from biological, economic, landscaping and cultural needs of each country that are realized using In situ and ex situ preservationincludingnational gene banks (NGB).
Introduction National Gene Bank should guarantee monitoring, collection and preservation of samples from genetic resources (spermatozoa, oocytes, embryos, somatic cells, tissues, DNA etc.) in original condition, deeply frozen or lyophilized; their storage, molecular-genetic characterization of samples, research-comparison of the genetic biodiversity, management of information system about stored samples and their use for original breed restoration.
Introduction In the last fifty years there was a significant decrease in the animal number in Slovak Republic and in some species the origin, autochthonous breeds completely disappeared. The possible extinction of these breeds would also mean irrecoverable loss of the genetic variability and so the loss of unique gene and allele combinations that would be very useful in the future e.g. for the generation of new farm animal genotypes. Wallachian sheep Slovak pinzgauer
Actual Animal Genetic Resorces in Slovak Republic 2015 EFABIS (http://efabis-sk.cvzv.sk)
Introduction • The Slovak Pinzgauer cattle, Wallachian sheep (Valaška), belong to these endangered breeds in Slovak republic.
Introduction • Oravka hen,Zobor or Nitra rabbit belong to these endangered breeds in Slovak republic
Animal Gene Bank in Nitra, Slovak Republic (Chrenek P. et al., Zygote 2013)
Fig. 4. Electron micrograph of transgenic (A) and non-transgenic (B) rabbit mammary gland tissues on D35 of third lactation. Each micrograph shows a supranuclear area with a higher presence of mitochondria (M) in the transgenic tissues. Protein globules (P) are seen in the lumen (L) of the alveolus in the non-transgenic tissue. Nucleus (N), Golgi apparatus (G), rough endoplasmic reticulum (ER), and display synthetic activity. Vacuoles (V) and microvilli (Mi) are seen. Magnification: x 12 800. b) a) Mi M V L Mi V M ER P ER G G Nc N N Analysis of rabbit transgenic and non-transgenic mammary gland Chrenek et al., AHE, 2010
Application of biotechnological method for cryopreservation of animal genetic resources 1) FAO database (http://efabis-sk.vuzv.sk) 2) DNA analysis (microsatelities) 3) Cryopreservation (Vitrification) of animal embryos, sperm and stem cells Nitra rabbit Zobor rabbit (Kulíkova B.,..,Chrenek P. et al., Theriogenology 2015)
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