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Gel Analysis of Plasmid Digestion with Enzymes NcoI, NheI, Ncol-HF, and Nhel-HF

This gel illustrates the digestion of plasmid pIS1-Eef25UTR-TOPmut-renilla using enzymes NcoI, NheI, Ncol-HF, and Nhel-HF, alongside the NEB 1kb ladder. The DNA was digested for 2 hours and run on a 0.8% agarose gel stained with ethidium bromide in TAE buffer for 2 hours at 60 volts.

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Gel Analysis of Plasmid Digestion with Enzymes NcoI, NheI, Ncol-HF, and Nhel-HF

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  1. This gel depicts the NEB 1kb ladder and a digestion of the plasmid pIS1-Eef25UTR-TOPmut-renillawith the following enzymes: NcoI, NheI, Ncol-HF + Nhel-HF Plasmid 38236 pIS1-Eef25UTR-TOPmut-renilla Ncol-HF +Nhel HF 1kb Ladder Ncol-HF Nhel-HF Uncut 0.5 1 1.5 2 3 4 5 6 8 10 DNA digested withNcoI, NheI, Ncol-HF+Nhel-HF for 2hours Gel: 0.8% TAE stained with 100 ml 1X TAE and 5uL of ethidium bromide Ran for2 hours at 60 volts

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