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Serial Dilution. 1 mL stock solution & 9 ml saline. 9 mL stock solution. 9 mL saline solution. 9 mL saline solution. 9 mL saline solution. 9 mL saline solution. 1 mL D2 solution to D3. 1 mL D3 solution to D4. 1 mL D4 solution to D5. 1 mL D5 solution to waste. D1. D2.

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## Serial Dilution

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**Serial Dilution**1 mL stock solution & 9 ml saline 9 mL stock solution 9 mL saline solution 9 mL saline solution 9 mL saline solution 9 mL saline solution 1 mL D2 solution to D3 1 mL D3 solution to D4 1 mL D4 solution to D5 1 mL D5 solution to waste D1 D2 D3 D4 D5 D6

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