Are You Sure You Want to Share That?. By: Sarah Akkoush and Sao-Mai Nguyen-Mau BSCI223H 0101 May 10, 2006. Background: Food and water as vehicles for transmission. Microbes can live in food and water
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By: Sarah Akkoush and Sao-Mai Nguyen-Mau
May 10, 2006
Approach: Consume different types of beverages in different volume intervals, taking a sample from each volume to be plated and analyzed.
Rationale: By taking this approach, it can be determined whether or not the amount of bacteria increased as the volume of liquid consumed increased. Also, the approach is conducive to determining whether or not the diversity of organisms present increased as the volume of liquid consumed increased.
For water and soda, a clear trend in number of bacteria is apparent. As volume of liquid consumed increased, number of bacteria on TSA plates increased. This is consistent with our expectation.
A clear trend in the number of organisms is not apparent for orange juice and milk. For orange juice, the cell count decreased as volume consumed increased. For milk, the number of organisms was hard to judge due to the expansive nature of the growth on the plates.
Sample at ¼.
Sample at ½.
Sample at ¾.
Sample at 7/8.
7/8 water sample on blood agar. Green circle represents beta-hemolysis. Black circles represent alpha-hemolysis.
Soda on blood agar. beta-hemolysis. Black circles represent alpha-hemolysis.
Gram negative cocci 1000x beta-hemolysis. Black circles represent alpha-hemolysis.
Gram positive cocci 1000x
Milk on blood. Majority of plate showed beta-hemolysis. beta-hemolysis. Black circles represent alpha-hemolysis.
Milk before consuming 1000x. beta-hemolysis. Black circles represent alpha-hemolysis.
Milk from sample at end of consumption 1000x.
Orange juice on blood agar. White circles are gamma hemolysis. Green circles denote beta hemolysis.
Colony that only appeared on ¾ sample of orange juice. hemolysis. Green circles denote beta hemolysis.
Gram stain of unknown colony on ¾ orange juice. 1000x hemolysis. Green circles denote beta hemolysis.
Capsule stain of orange juice colony. 1000x hemolysis. Green circles denote beta hemolysis.
Alpha-hemolytic colony on blood agar of water 1000x hemolysis. Green circles denote beta hemolysis.
Gamma-hemolytic colony on blood agar of orange juice 1000x
Beta-hemolytic colony 1000x.
Gram stain of brown colony on soda agar plate 1000x. hemolysis. Green circles denote beta hemolysis.
Gram stain of white colony on soda agar plate 1000x
Pneumonia: hemolysis. Green circles denote beta hemolysis.
Inflammation of the lungs do to microbial infection.
Can be acquired through contact with an infected person and airborne microbes.
Symptoms include: shaking chills, a high fever, sweating, chest pain (pleurisy) and a cough that produces thick, greenish or yellow phlegm
Prevention: take care of yourself, wash your hands, don’t smoke, don’t infect others
Strep throat: hemolysis. Green circles denote beta hemolysis.
An infection of the upper respiratory tract, specifically the pharynx.
Symptoms: Throat pain, difficulty swallowing, red and swollen tonsils, sometimes with white patches or streaks, swollen, tender lymph glands in the neck, fever, headache
Treatment: antibiotics, such as penicillin, amoxicillin, and azithromycin. Unfinished prescriptions can lead to rheumatic fever.
A.D.A.M. Medical Illustration Team 2004
Prevention: wash your hands and do not share drinks with infected person
Diphtheria hemolysis. Green circles denote beta hemolysis.
Contagious infection of the throat and nose. Symptoms are caused by toxins secreted by the microbe. Transmission is through contact with infected individuals.
Symptoms: sore throat and mild fever, membrane forms around throat and tonsils, lymph glands and tissue on both sides of the neck to swell a large size.
Treatment: antibiotics (erythromycin) and antitoxins
Virtual Pediatric Hospital
Prevention: diphtheria vaccine
Significance of ‘backwash’: hemolysis. Green circles denote beta hemolysis.
possible transmittance of pathogenic bacteria, such as S. pneumoniae, S. pyogenes, and C. diphtheriae.
growth of bacteria from samples in experiment indicates that oral bacteria can be transferred to a beverage
if that beverage is shared, the bacteria can enter another person and cause disease
Bibliography hemolysis. Green circles denote beta hemolysis.
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Kohn, W., Collins, A., Cleveland, J., Harte, J., Eklund, K., and Malvitz, D. “Guidelines for Infection Control in Dental Health-Care Settins – 2003.” Morbidity and Mortality Weekly Report 52(RR17). 2003. Center for Disease Control and Prevention. 25 April 2006. http://www.cdc.gov/mmwr/preview/mmwrhtml/rr5217al.htm.
Madigan, M. and Martinko, J. Brock Biology of Microorganisms. Upper Saddle River: Pearson Education, Inc. 702.
“ hemolysis. Green circles denote beta hemolysis. Meningococcal Disease.” Division for Bacterial and Mycotic Diseases. 2005. Centers for Disease Control and Prevention. May 2006 http://www.cdc.gov/ncidod/dbmd/diseaseinfo/meningococca l_g.htm #Is%20meningitis%20contagious.
Prokaryotes. Springer New York, 2004. http://184.108.40.206:8080/prokPUB/index.htm.