Polyherbal Formulations for Diabetes

1. Polyherbal Formulations for Diabetes Prof. Dr. Basavaraj K. Nanjwade. M.Pharm., Ph.D. Department of Pharmaceutics KLE University College of Pharmacy Belgaum, Karnataka , India E-mail: bknanjwade@yahoo.co.in Cell No: 00919742431000

2. Graphical Abstract 14th ACC and ANRAP, Bangkok

3. Introduction • Plant formulation and combined extracts of plants are used a drug of choice rather than individual. Various herbal formulations such as diamed,coagent db, Diasulin, and hyponidd, are well known for their antidiabetic effects. • Polyherbal formulation of Annona squamosa and Nigella sativa is composed of medicinal plants (Table 1), which are traditionally used for antidiabetic and antihyperlipidemic activity. The present investigation was undertaken to study the effect of the polyherbal formulation of Annona sqamosa and Nigella sativaon lipidperoxidation and tissue lipid profile in streptozotocin induced diabetic rats. 14th ACC and ANRAP, Bangkok

4. ResearchBackground • Polyherbal formulation of Annona squamosa and Nigella sativa on blood glucose, plasma insulin, tissue lipid profile, and lipidperoxidation in streptozotocin induced diabetic rats. Aqueous extract of Polyherbal formulation of Annona squamosa and Nigella sativa was administered orally (200 mg/kg body weight) for 30 days. • The different doses of Polyherbal formulation on blood glucose and plasma insulin in diabetic rats were studied and the levels of lipid peroxides and tissue lipids were also estimated in streptozotocin induced diabetic rats. The effects were compared with tolbutamide. Treatment with Polyherbal formulation and tolbutamide resulted in a significant reduction of blood glucose and increase in plasma insulin. 14th ACC and ANRAP, Bangkok

5. Annona squamosa (Sugar–Apple) 14th ACC and ANRAP, Bangkok

6. Nigella sativa 14th ACC and ANRAP, Bangkok

7. Material and methods • Animals • Preparation of drug • Chemicals • Drug administration • Streptozotocin-induced diabetes • Experimental design • Biochemical analysis 14th ACC and ANRAP, Bangkok

8. Experimental design • In the experiment, a total of 42 rats (30 diabetic surviving rats, 12 normal rats) were used. • The rats were divided into seven groups of six rats each after the induction of streptozotocin diabetes. 14th ACC and ANRAP, Bangkok

9. Experimental design • Group1: Normal treated rats • Group 2:Normal rats given aqueous solution of Polyherbal formulation (200 mg/kg body weight) daily using an intragastric tube for 30 days. • Group 3:Diabetic control rats. • Group 4: Diabetic rats given aqueous solution of Polyherbal formulation (50 mg/kg body weight) daily using an intragastric tube for 30 days. 14th ACC and ANRAP, Bangkok

10. Experimental design • Group 5: Diabetic rats given aqueous solution of Polyherbal formulation (100 mg/kg body weight) daily using an intragastric tube for 30 days. • Group 6: Diabetic rats given aqueous solution of Polyherbal formulation (200 mg/kg body weight) daily using an intragastric tube for 30 days. • Group 7: Diabetic rats given aqueous solution of Tolbutamide (250 mg/kg body weight) daily using an intragastric tube for 30 days. 14th ACC and ANRAP, Bangkok

11. Biochemical analysis • Estimation of blood glucose and plasma insulin • Estimation of lipid peroxidation • Estimation of lipids i. Lipids ii. For total cholesterol estimation iii. Fro triglycerides estimation iv. Phopholipids content v. Free fatty acids vi. Statistical analysis 14th ACC and ANRAP, Bangkok

12. Table 1: Polyherbal Formulation of Annona Squamosa and Nigella sativa (Composition and Concentration) 14th ACC and ANRAP, Bangkok

13. Table 2: Changes in blood glucose and plasma insulin levels of control and experimental animals 14th ACC and ANRAP, Bangkok

14. Table 3: Changes in levels of TBARS and Hydroperoxides in liver and kidney of control and experimental animals 14th ACC and ANRAP, Bangkok

15. Table 4: Changes in levels of cholestrol, free fatty acids, triglycerides and phospholipids in liver of control and experimental animals ( mg/100g wet. tissue) 14th ACC and ANRAP, Bangkok

16. Table 5: Changes in levels of cholestrol, free fatty acids, triglycerides and phospholipids in kidney of control and experimental animals ( mg/100g wet. tissue) 14th ACC and ANRAP, Bangkok

17. Conclusion • Polyherbal formulation of Annona squamosa and Nigella sativa, exert a significant antihyperlipidemic. This could be due to combined effect of Annona squamosa and Nigella sativa. Hence the antihyperlipidemic effect of polyherbal formulation of Annona squamosa and Nigella sativa in particular could be considered as of possible therapeutic value 14th ACC and ANRAP, Bangkok

18. Liposomes • Spherical vesicles with a phospholipid bilayer Hydrophilic Hydrophobic Hydrophobic 14th ACC and ANRAP, Bangkok

19. 14th ACC and ANRAP, Bangkok

20. Liposome Preparation 14th ACC and ANRAP, Bangkok

21. Liposome Preparation 14th ACC and ANRAP, Bangkok

22. Liposome Preparation 14th ACC and ANRAP, Bangkok

23. Lipid Peroxidation • Most phospholipid liposomes contain unsaturated acyl chains as part of their molecular structure and susceptible to oxidative degradation. It can be minimized by the use of animal derived lipids like egg PC, which has less saturated lipids, use of light resistant containers, use of antioxidants are useful in minimizing oxidation. 14th ACC and ANRAP, Bangkok

24. Diabetic Current Research • Mangifera indica(stem,fruits,etc) – Anacardiaceae – Mango • Gossypiumherbaceum(flowers,etc ) – Malvaceae – Cotton • Cocos nucifera(roots,etc) – Arecaceae – Coconut • Lawsonia inermis(bark,etc) – Lythraceae - Mendhi 14th ACC and ANRAP, Bangkok

25. My Research Group 14th ACC and ANRAP, Bangkok

26. THANK YOUE-mail: bknanjwade@yahoo.co.inCell No: 0091 9742431000 14th ACC and ANRAP, Bangkok