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Validation Study of Two Rapeseed Oil Body Extraction Methods. Stephanie BLASER Marc ANTON, Eisabeth DAVID-BRIAND, Thibault LOISELEUX INRA 18/07/2014. Introduction. Rapeseed Brassica napus Common uses Bulk oil - canola oil High protein a nimal feed Biodiesel. Introduction.

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validation study of two rapeseed oil body extraction methods
Validation Study of TwoRapeseedOil Body Extraction Methods

Stephanie BLASER

Marc ANTON, Eisabeth DAVID-BRIAND, Thibault LOISELEUX

INRA

18/07/2014

introduction
Introduction
  • Rapeseed
    • Brassicanapus
    • Common uses
      • Bulkoil - canola oil
      • High proteinanimal feed
      • Biodiesel
introduction1
Introduction
  • Oil bodies
    • Energystorage structures in plant seeds
    • 0.5- 2.5 µm diameter
    • Components
      • Triaclyglycerolcore
      • Outer phospholipidmonolayer
      • Charged surface proteins - oleosins
    • Structure givesphysical and chemicalstability
introduction2
Introduction
  • Potential applications in the humanfood system
    • Deliver stable, preemulsifedoilintoappropriatefoodsystems
    • Create a poorly-digestedemulsion, through high pressure processing, for the increasing obese population
    • Have an oil source extractedwithoutsolvents
  • Objective
    • Find the methodthatextracts the highestquantity of oil bodies with the highestpurity
materials and methods
Materials and Methods
  • Extraction Method 3
materials and methods1
Materials and Methods
  • Extraction Method 5
materials and methods2
Materials and Methods
  • Extraction 3 and 5 treatments
    • Regular - freshcream in phosphate buffer (100 mg/ml)
    • Freeze-driedcream in phosphate buffer
    • Freeze-driedcream in water
  • Analyses
    • Dry Matter
    • Size
      • Microscope
      • Granulometer
      • Nanosizer
    • Protein quantification
      • BSA
    • Lipid quantification
      • Isopropanol/hexane extraction
light microscope
Light Microscope

Method 3

Method 5

63x

63x

nanosizer method 3
Nanosizer – Method 3

Regular

Freeze-dried

nanosizer method 5
Nanosizer – Method 5

Regular

Freeze-dried

discussion
Discussion
  • Cream Collection Weights
    • No major differencebetween Method 3 and 5
  • Size
    • Method 3 vs 5
      • Bothdisplayedsimilarpresence of floculation and particle sizes- granulometer, nanosize, and light microscope
    • Regular vs. Freeze-dried
      • Light microscope
        • No major visualdifferences
      • Granulometer
        • More floculation present in samplesfreeze-driedwithbuffer
      • Nanosizer
        • Freeze-driedsamplesshoweda wider range of particle sizes
      • Differences in freeze-driedsamplespotentiallydue to destruction of oil bodies duringharshtreatment
discussion1
Discussion
  • Lipid Quantification
    • Slightlyhigher collection from Method 3
  • Protein Quantification
    • Slighlylower concentration from Method 3
  • Dry Matter
    • Slightlyhigher Percent Dry Matterfrom Method 3
discussion2
Discussion
  • Challenges
    • Cream collection
      • Cream can stick to cap of centrifuge tube
      • Cream in Method 3 is, in general, not as firm and durable as Method 5
    • Size measurement instrumentation
      • Granulometer and nanometer have size detectionlimitsthat are on bothsides of the oil bodies upper and lowerdiameter range
      • New equipmentarrivingnextmonth
    • Freeze-drying
      • Low volume of final product
      • Time consuming
      • Slightlylowerprotein content
      • With buffer
        • Powdery final product - easier to collect
        • Contained phosphate buffer salts
          • Room for errors in calculations
      • With water
        • Waxy final product - more difficult to collect
sustainability
Sustainability
  • Future options
    • Usingsustainabiliygrownseeds
      • 2010 Unilever Sustainable Agriculture Code
        • Put intoeffect in Germany under Cargill
    • Using the valueableprotein for a humanfood source and not just for animal meal
      • Hurdles: glucosinolates, phenolics, phytates, and high amount of fiber
      • Benefits: balanced amino acid profile, functional properties (emulsifying, foaming, and gelling), and new alternative to feed increasing population
    • Creatingindustrialscale extraction methodswithout the use of dangeroussolvents
conclusion
Conclusion
  • Method 3
    • More consistent
    • Less time consuming
    • Lessprotein contamination
  • Method 5
    • Easier handling of cream
    • Long soakstep
    • More protein contamination
  • Freeze-drying
    • Time consuming
    • Few addedbenefits
  • Nextstep
    • Compare againstThibault’s data
    • Increase collection volume to largerbenchscale
thank you
Thank You

Stephanie Jung, PhD

Marc Anton, PhD

Elisabeth David-Briand

Thibault Loiseleux

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