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Pst DC3000 1 2 3. 1= vector control 2= AvrRpt2 100 -AvrBsT 32-350 -HA 3= AvrRpt2 100 -AvrBsT 32-350 -(H154A)-HA. Pi-0 HR- HR+ HR- Col-0 HR- HR- HR-.

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slide1

Pst DC3000

1 2 3

1= vector control

2= AvrRpt2100-AvrBsT32-350-HA

3= AvrRpt2100-AvrBsT32-350-(H154A)-HA

Pi-0

HR- HR+ HR-

Col-0

HR- HR- HR-

Supplementary Figure 1. Pst DC3000 expressing the AvrBsTHyb catalytic core mutant protein does not elicit a HR in Arabidopsis Pi-0 leaves. Pi-0 and Col-0 leaves were hand-inoculated with a 3 x 108 cfu ml-1 suspension of Pst DC3000 carrying pDD62, or pVSP61(avrRpt21-100-avrBsT32-350-HA), or pVSP61(avrRpt21-100-avrBsT32-350-(H154A)-HA). Symptoms were photographed 12 h post-inoculation.