Conventional SEM. Specimen at high vacuum – requires sample fixation and dehydration or freezing. Charging is minimized by coating sample with metal or carbon or lowering the operating kV. Variable Pressure Scanning Electron Microscope. Variable Pressure Scanning Electron Microscope.
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Specimen at high vacuum – requires sample fixation and dehydration or freezing.
Charging is minimized by coating sample with metal or carbon or lowering the operating kV.
When the electron beam strikes a sample,
both photon and electron signals are emitted.
- composition info
- Atomic number and topographical
- Surface sensitive compositional
Incident Electron Beam
Zeiss VPSE Detector Principle
The photons are detected and amplified to provide the final image.
Heats to 50 C
Cools to - 25 C
Bacteria on rock
X-ray imaging that reconstructs images to form cross-sections and volumetric information.
Resolution to 5 mm, 3D reconstruction, density measurements.
Any sample works having differential density within sample (e.g. bone vs. tissue, or addition of x-ray contrast agents)
Applications – Bone, insects, food science, material science, substrate/cell distribution.
Object is rotated 180 degrees.
Images captured at one degree increments.
Reconstructions done on aligned images to create volume data.