1 / 24

External Advisory Board

The NHLBI Specialized Center of Clinically Oriented Research (SCCOR) in Pediatric Heart Disease at the Children's Hospital of Philadelphia: P50-HL74731 Program Title: Genetic Mechanisms in Pediatric Heart Disease http://stokes.chop.edu/programs/sccor/ Program Director: Robert J. Levy, M.D.

ciara
Download Presentation

External Advisory Board

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. The NHLBI Specialized Center of Clinically Oriented Research (SCCOR) in Pediatric Heart Disease at the Children's Hospital of Philadelphia: P50-HL74731Program Title: Genetic Mechanisms in Pediatric Heart Diseasehttp://stokes.chop.edu/programs/sccor/Program Director: Robert J. Levy, M.D.

  2. External Advisory Board Elazer R. Edelman, MD, PhD, FACCDirector, Harvard-MIT Biomedical Engineering CenterThomas D. and Virginia W. Cabot ProfessorHealth Sciences and TechnologyMassachusetts Institute of Technology & Harvard Medical School, Attending Cardiologist, Brigham and Women’s Hospital David H. Ledbetter, Ph.D., Robert W. Woodruff Professor of Human GeneticsDirector, Division of Medical Genetics Emory University School of Medicine Jurg Ott, Ph.D. Professor and Head Laboratory of Statistical Genetics Rockefeller University

  3. The CHOP SCCOR—Programmatic Hypothesis “The CHOP SCCOR is a direct outgrowth of productive research at our Institution over the past decade that was based on the hypothesis that congenital heart abnormalities are caused by gene defects” “Basic discoveries concerning gene abnormalities and related patterns of gene expression can be applied to a unifying approach for both understanding the complex basis for cardiac dysmorphogenesis as well as providing therapeutic insights for translational directions.”

  4. THE CHOP SCCOR GENETIC MECHANISMS IN PEDIATRIC HEART DISEASE

  5. Project 1:Biocompatible Heterograft Biomaterials • Investigations concerning novel surgical therapies for congenital cardiac malformations. • Project Leader: Robert J. Levy, M.D., Professor of Pediatrics and Pharmacology, University of Pennsylvania School of • Medicine • Specific Aims • Triglycidyl Amine (TGA)—A new crosslinking reagent for preparing heart valve bioprostheses: Chemical and biological mechanisms • 2. TGA-Matricellular interactions: Cellular and molecular biology studies related to anticalcification mechanisms. • 3. Mechanisms responsible for TGA-mediated inhibition of heart • valve calcification: Biomechanics, biocompatibility, & changes in gene expression patterns. • Subcontract PI: Joseph Gorman, M.D., Asst.Prof. Surg. Univ.Penn Sch.Med. • Subcontract PI: Michael Sacks, Ph.D., Prof. of Bioengineering, Univ.Pitt.

  6. Bioprosthetic Heart Valves Carpentier- Edwards Hancock [Medtronic] Ionescu-Shiley Porcine aortic valve Porcine aortic valve Bovine pericardium

  7. Calcified Bioprosthesis

  8. Project 1 Triglycidyl Amine (TGA) • Reacts irreversibly with lysine, methionine, cystine, histidine • Results in biomechanical properties superior to glutaraldehyde • Biocompatibility—supports cellular growth of all cardiovascular cell types

  9. Project 2:Genetic Analysis of Human Outflow Tract Malformations • Project Leader: Deborah Driscoll, M.D., Professor and Chair, • Department of Obstetrics and Gynecology, University of Pennsylvania School of Medicine • Specific Aims • Developing a map of single nucleotide polymorphisms (SNP’s) in selected genes implicated in cardiac development. • 2. Evaluating SNP’s for potential functional alterations. • 3. Determining the genetic contribution of selected genes to the development of outflow tract malformations using family-based linkage disequilibrium testing. • 4. Identifying modifiers of the cardiac phenotype in patients with 22q11 deletions

  10. VEGF Related Directions • Animal models demonstrate VEGF capable of influencing pharyngeal arch patterning • SNPs with reduced VEGF expression are associated with cardiovascular defects in 22q11 deletion syndrome • Identified an “at risk” haplotype for cardiovascular defects among individuals with 22q11 deletion • Suggests risk of CHD in the fetus with 22q11 deletion increases when VEGF levels fall below critical threshold needed for proper development of pharyngeal arch arteries, severity may be due to degree of vascular impairment

  11. Project 3:Genotype and Clinical Outcome in Conotruncal Defects • Project Leader: Elizabeth Goldmuntz, M.D., Associate Professor of Pediatrics, University of Pennsylvania School of Medicine • Specific Aims • Investigations of the contribution of NKX2.5 and related genes to the etiology of controtruncal defects using mutation analyses and family-based association studies. • 2. Investigating whether subsets of patients with transposition of the great arteries or double outlet right ventricle share a common genetic etiology with the heterotaxy syndrome: Studies of CFC1 mutations & other genes (NODAL, ZIC3,LEFTY1, ACVRIIB) • 3. Studies of the relationship between genetic etiology and clinical variability/outcome in subjects with conotruncal defects

  12. Project 3: Aim 3 Impact of Genotype on Clinical Status • Cross sectional study • Subjects with TOF, Truncus or IAA • Ages 8-18 yo • Clinical Assessment • Exercise study • Echocardiogram • Cardiac MRI • Child health questionnaire

  13. Project 4:Molecular Analysis of Human Subtelomeric Rearrangements • Project Leader: Ian Krantz, M.D., Assistant Professor of Pediatrics and Genetics, University of Pennsylvania School of Medicine • Specific Aims • Identify individuals with subtelomeric chromosomal deletions and congenital heart defects. • 2. Develop a diagnostic assay that targets the critical region and sizes subsequent rearrangements of each of the telomeres. • 3. Defining the critical regions and identifying candidate disease related genes for specific clinical phenotypes by mapping the extent and composition of the associated rearrangements.

  14. Project 5:Chromosomal Rearrangements (CR) and Cardiac Candidate Genes Project Leader: Beverly S. Emanuel, Ph.D., Professor and Chair of Genetics (at CHOP), University of Pennsylvania School of Medicine Specific Aims 1. Identify and characterize CR’s in patients with congenital heart disease by high-resolution cytogenetics and molecular cytogenetic analysis 2. Develop PCR-based mapping strategies using the human genomic sequence to identify the translocation BP’s 3. Characterize the genomic DNA from normal chromosomes at the chromosomal breakpoints in order to identify mechanisms of rearrangement 4. Identify the candidate genes disrupted or deleted at the translocation BP’s as candidates for early cardiac morphogenesis. 5. Determine whether mutations in the candidate genes are associated with the specific cardiac defect in other patients in the SCCOR Clinical Core.

  15. The Clinical Core (a continuing resource from the SCOR’s) • Director: Elizabeth Goldmuntz, M.D. • Objectives: • Molecular analyses of the genetic etiology of • conotruncal defects • 2. Molecular analyses of bioprostheses • 3. Impact of genotype on cardiac anatomy and clinical outcome • Services • Ascertain Subjects • Acquisition of clinical data • Acquisition of relevant samples • Review of pertinent medical records • Coordination of clinical studies

  16. The Cell Culture, DNA, and Microarray Core • (a continuing resource from the SCOR’s) • Director: Beverly Emanuel, Ph.D. • Objectives: • Provide cell culture, DNA isolation, cytogenetic and • DNA analysis support for all of the projects • 2. Provide microarray resources • 3. Training and consultation services to all Projects and • Cores • Services: • Establishing lympholastoid cell lines from patients • with congenital heart defects. • 2. Isolation of DNA from established cell lines, peripheral lymphocytes • 3. Perform FISH to screen for 22q11.2 deletions • 4. Regionally localize newly identified human cDNAs by FISH • 5. Provide genotyping services for the SCCOR

  17. Cardiac Morphology, Gene Expression and Histology Core • Director: Kenneth Ryan, Ph.D., Assistant Professor of Pediatrics, • University of Pennsylvania School of Medicine • Objectives: • Analysis of gene (mRNA and protein) expression • 2. Histological support • Breed mice & xenopus for harvesting embryos for whole-mount • in situ hybridizations and sectioning re. cardiac gene expression patterns • Services • 1. Generate and bank frozen staged mouse & xenopus embryo RNA • samples. • 2. Generate as blocks and slides embedded mouse and xenopus • embryos. • 3. Share expertise in and perform in situ hybridizations using antisense • RNA probes. • 4. Perform immunohistochemistry and related histology

  18. Bioinformatics and Data Analysis Core • Director: Peter White, Ph.D., Assistant Prof. Ped.,Univ.Penn.School • of Medicine, Director, CHOP’s Bioinformatics Core • Co-Director: Charles Scott, Ph.D. • Objectives: • State of the art bioinformatics & biostatistics resources • 2. Experimental design support • Data base development and data management support • The integration of bioinformatics and biostatistics data • on SCCOR subjects • Services • 1. Provide assistance in study design, database design, • and data storage • 2. To provide infrastructure, hardware, software, technical support, • for analyzing results of molecular biology experiments • 3. Statistical analysis and data interpretation

  19. NHLBI PEDIATRIC HEART DISEASE SCCOR’s: 2005 Programmatic Meeting At the NHLBI, December 13, 2005 Children’s Hospital, Boston PI: Jane Newburger, M.D. Children’s Hospital of Cincinnati PI: Woody Benson, M.D., Ph.D. Children’s Hospital of Pittsburgh Steve Webber, M.D., Ph.D.

  20. From Molecular Mechanisms to Improved Outcomes in TOF Jane W. Newburger, M.D., M.P.H. Children’s Hospital, Boston Harvard Medical School

  21. Children’s Hospital, Boston:Projects Project 1: Neurologic and developmental outcome in TOF (Newburger) Project 2: Randomized trial of pulmonary valve replacement in TOF (Geva) Project 3: Human mutations that cause TOF (Seidman) Project 4: Mitochondria in hypertrophied RV and surgical ischemia (McGowan) Project 5: Functional analysis of cardiac transcription factor NKX2.5 (Izumo/Jay) Project 6: Cardiac regeneration in zebrafish (Keating)

  22. Cores Core A: Research support and statistics (Newburger) Core B: Microarray core (Schinke) Core C: Children’s Hospital-Harvard TOF registry (Breitbart) Core D: Skills development core: The pathology of CHD (Collins/Jurazek)

  23. Children’s Hospital of Cincinnati: SCCOR in Pediatric Heart Disease: PI Woody Benson “Molecular mechanisms of valve development and disease” PI: D. Woodrow Benson, MD, PhD Project 1 – Benson—Genetic Studies of Valvular Heart Disease Project 2 – Gelb—To Identify PTPN11 Defects Associated with Noonan’s syndrome and other forms of congenital heart disease. Project 3 – Yutzey--Regulation of valvuloseptal development by DSCR1 Project 4 – Robbins-- Mechanisms of Cardiac pathogenesis in Noonan Syndrome, effects of SHP-2Gln79Arg, a common PTPN11 mutation associated with Noonan’s syndrome.

  24. SCCOR Program S.A. Webber, PI Consultants PROJECTS CORES Project 1 Thymic Tolerance S. A. Webber, PI Administration S. A. Webber, PI Project 2 Transplant EBV Disease D. T. Rowe, PI Immunological Monitoring D. Metes, PI Project 3 Genetic Contributions to Transplant Outcomes A. Zeevi, PI Biostatistics and Data Management S. Kelsey, PI Clinical Skills Training B. B. Keller, PI Children’s Hospital of Pittsburgh: Optimizing Outcome after Pediatric Heart Transplantation

More Related