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Binge cocaine treatment / Withdrawal day 1 / D2R . Saline. Cocaine. NS. counts /min. CPu. 100. 80. Nac. 60. 40. 20. 0. Saline. Cocaine. NS. counts /min. 70. 60. 50. 40. SN. VTA. 30. 20. 10. 0.

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slide1

Bingecocainetreatment / Withdrawalday 1 / D2R

Saline

Cocaine

NS

counts/min

CPu

100

80

Nac

60

40

20

0

Saline

Cocaine

NS

counts/min

70

60

50

40

SN

VTA

30

20

10

0

Supplemental Figure 2: Computer-enhanced autoradiograms of coronal brain sections from saline or cocaine-treated mice showing different region of interest.

Brain sections were incubated with [3H]-Raclopride as described in material and methods and radioactivity was detected using beta imager. (CPu, caudate putamen; Nac, nucleus accumbens; SN, substantianigra; VTA, ventral tegmental area; NS: non specific binding)