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Disruptive Technologies Product Range and Manufacturers Represented. Biolgical Sample Preparation Nucleic acides, protein and small molecules extraction from hard-to-lyse tissue samples (Pressure Biosciences) Dissolution/Formulation

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slide2

Disruptive Technologies Product Range and Manufacturers Represented

  • Biolgical Sample Preparation
    • Nucleic acides, protein and small molecules extraction from hard-to-lyse tissue samples (Pressure Biosciences)
  • Dissolution/Formulation
    • Dissolution baths, friability and disintegration instruments (Distek)
  • Physico Chemistry
    • HT Log P and pKa analyzer (AATI)
    • Kinetic solubility instruments (Analiza)
  • Rapid microbiology with MicroPRO (AATI)
  • Service
    • Agilent Channel Partner to service their range of HPLC, UV spectrophotometers and CE systems
  • Preparative
    • OPLC chromatography solutions for semi preparative applications (OPLC systems, pumps, sample applicator, video imaging and densitometry instruments, reagent sprayer (OPLC-NIT)
    • Flash chromatography (Gyan)
    • Automated SPE system (HTA)
  • Analytical
    • HT oligonucleotides purity analyzer (AATI)
    • HT proteins analyzer (AATI)
    • HT DNA analyzer (AATI)
    • HT Chiral analyzer (AATI)
    • Spotter for MALDI and tissue MALDI imaging (SunChrom)
    • Type-C silica hydride HPLC columns Flat sorbent beds for OPLC (MicroSolv)
    • Accessories and consumables for CE and HPLC (MicroSolv)
    • Validation kits for HPLC systems (MicroSolv)
who are we
Who are we?

Our History: Founded 1997, 4 Scientific Co-founders, Privately Funded

Our Business: Rapid Microbial Detection Technology

Capillary Electrophoresis (January 2007)

Our Markets: Pharmaceutical, Personal Care Products, Fermentation, Environmental, Drug Discovery, Oligonucleotides Production.

Our Solution: Replace current microbial detection methods (requiring 24 – 72 hours). High throughput quality analysis of oligonucleotides and fast pKa and Log P determinations of chemical compounds.

Our Products: MicroPRO Instrument, Custom Kits. OligoPRO and pKa PRO analyzers.

overview of microbial detection presentation
Overview of Microbial Detection Presentation
  • How the Technology Works
  • Standard Labeling Protocol
  • The MicroPRO Instrument
  • Water Monitoring
  • Bacteria, Yeast and Mold in 24 hours in Products
  • Surface/Environment Monitoring Using Swabs
  • Fermentation Products
  • Challenge Tests in Products
basics of flow cytometry1
Basics of Flow Cytometry
  • Laser-based irradiation of cells
  • Fluorochromes bound to cells provide information on cell state (e.g., live, dead, spores, vegetative)
  • Light scattering provides relative size information
  • System composed of fluidic, optic and electronic components
  • Advantages: Rapid and quantitative analysis of individual cells
slide8

Fluidic System

Sample delivery

(0.05mL/min)

Sheath delivery

(16-18mL/min)

  • Quantitative cell delivery
  • Hydrodynamic Focusing
  • Single File Passage through detection region

Sheath flow

Labeled bacteria

Core flow

slide9

Optic System

Labeled microorganism

Scatter

Detector

Scatter signal

Laser Beam – shaped and focused;

635 nm

laser excitation

Fluorescence

Detector

Fluorescence

signal

High performance

optical filters

Fluorescence plus Scatter = One Count

electronic system
Electronic System
  • Signal processing component
  • The Micro PRO™ triggers on fluorescence
  • Fluorescent event above the threshold is processed, along with the corresponding scatter event, and is plotted and recorded as a count

= 1 count

Detector

output

= 0 count

Fluorescence Threshold Level

Time

signal processing
Signal Processing

Microbe A

cumulative

Microbe A + B

Microbe A + B +C

slide13

Results

Fluorescence Intensity vs. Counts

Intensity Plot

Scatter Intensity vs. Counts

Box = 9907 counts/0.25mL

97.4% of the counts are in the box

slide15

RBD Total

Cell Count / mL

Biomass Labeling Protocol

(Membrane Permeable – all Cells are Stained)

  • Ideal for enumerating Live & Dead microbes in:
    • Fermentation cultures
    • Spore preparations
slide16

Ideal for enumerating Viable microbes in:

    • Process water monitoring
    • Presence/Absence testing
    • Fermentation cultures
  • Final product testing
  • Pure cultures

TVO Labeling Protocol

(Only Viable Cells are Stained)

slide17

Antibody-Specific Detection

Labeling Protocol

Mixed Sample

  • Ideal for enumerating microbes for/in:
    • Specific pathogen detection
    • Mixed cultures
    • High background matrices
slide20

Auto-Sampler

Auto-Prep

  • Holds up to 42 samples
  • Adds reagents
  • Mixes sample
  • On-board automated cleaning and bubble removal
analysis on the micro pro
Analysis on the Micro PRO™

2

1

Load sample vials and syringes

Select Tray SetUp

Reagent additions and sample injection performed automatically as defined in the Method

Micro PRO™ Output:

Pass/Fail

&

Counts/mL

3

4

sample preparation
Sample Preparation
  • Collect sample;
  • Dispense 3mL sample into 5mL snap-cap tube
  • Load sample into MicroPRO Sample Tray with capped syringe
  • Select pre-defined (or create new) Analysis/Tray Sequence
  • MicroPRO count result in 5 minutes
slide25

1

2

3

Background / Neg.Ctrl Sample 3 Sample 7

Box = 0 counts/0.25mL Box = 2 counts/0.25mL Box = 22 counts/0.25mL

purified water system
Purified Water System

From: Hasher-Homesley, P.1, 2006. R&D Applications for the RBD3000.

1Johnson & Johnson Vision Care. Rapid Microbial Methods User’s Meeting, Chicago, IL

product research lab water testing
Product Research Lab Water Testing

Kozak, K.1, 2006. Rapid Microbiological Testing in Support of Product Development.

1Procter and Gamble. Rapid Microbial Methods User’s Meeting. Chigaco, IL

traditional methods usp 61
Traditional methods USP <61>

Bacteria

1:10 dilution of product

1 ml in each of two Petri dishes with Soybean Casein Digest medium melted <45 C

Incubate 48 to 72 hours at 30 C

Count

If zero counts, results are expressed as less than 10 cfu/ml

Yeast and Mold

1:10 dilution of product

1 ml in each of two petri dishes Saboraud Dextrose Agar

Incubate 5 to 7 days at 20-25 C

Count

If zero counts, results are expressed as less than 10 cfu/ml

slide33

Advanced Analytical Method

Bacteria, Yeast & Mold

Incubate 24 to 48hours at 30°C

Transfer substrate tube A to

Tube B; vortex; 0.1mL to Tube C

1:10

dilution of

product

1ml in Tube A

Micro PRO™

slide34

Product Test Kit

35mm filter

mold

Micro PRO™

Transfer swab

from Tube A to B

Tube A - GEM

Add product, enrich

Tube B - PB

Add swab, mix

Tube C - PB

Add 0.1mL from Tube B

slide35

Experimental Procedure

  • Prepare 1:10 product suspension in buffer or neutralizing growth enhancement media
  • Add 1mL 1:10 product suspension to Tube A & neutralize 30 min
  • Spike with <100 cfu Escherichia coli ATCC 8739 or 25922, Pseudomonas aeruginosa ATCC 9027, Staphylococcus aureus ATCC 6538, Candida albicans ATCC 10231, Aspergillus niger ATCC 16404
  • Prepare non-spiked product controls
  • Enrich at 30oC for 24-48 hours
  • Transfer substrate from Tube A to Tube B and vortex
  • Transfer 0.1mL from Tube B to Tube C and load in the Micro PRO™
  • Plate samples post-enrichment
analysis on micropro
Analysis on MicroPRO

2

Select Method

Load sample vials and syringes

1

Reagent additions and sample injection performed automatically as defined in the Method

Results

3

4

slide38

Results

Micro PRO™ output shows few to no counts within the area definitions (product baseline)

Pass result indicates that the sample does not contain microbial contamination

slide39

Results

Micro PRO™ output shows many counts within the area definition (>3X product baseline)

Fail result indicates that the sample contains microbial contamination

Bacteria

slide40

Yeast

Mold

slide44

Negative Control

Positive

Ps. aeruginosa

slide45

Negative Control

Positive C. albicans

slide46

Negative Control

Positive A. niger

results
Results
  • Approximately 300 samples were run parallel to standard cultural methods. Results from RBD are equivalent to the plate method.
the advanced analytical solution provides
The Advanced Analytical Solution Provides
  • One test for the detection of bacteria, yeast & mold in a variety of matrices
  • Rapid screening for microbial contamination with 24 hour results for a majority of the products tested
  • Objective Pass/Fail results requiring no additional interpretation
  • Pass/Fail criteria that generate no false positives or false negatives
  • Versatile system for quantification of microorganisms in purified water, surface swabs, and pure cultures
environmental swab protocol
Environmental Swab Protocol
  • Place a swab sample in a 5mL snap-cap tube containing 900µL filtered, sterile PB
  • Break the swab handle over the rim of the tube
  • Replace snap-cap and vortex swab and buffer for 30 seconds
  • Press the swab against the side of the tube to express extra liquid
  • Bring the volume to 3mL with filtered, sterile PB
  • Analyze samples and controls on the MicroPRO
slide51

1

2

3

4

environmental sample testing
Environmental Sample Testing
  • Swab samples are directly analyzed on the MicroPRO
  • Result is obtained in minutes rather than overnight

Swab Control

Box counts/0.25mL: 2

E.coli Swab

Box counts/0.25mL: 1,195

Swab Sample: Population

indicative of residual product

Residual product; an additional box may be created to capture this data

Microbial population within a predefined box

environmental sample testing1
Environmental Sample Testing
  • Surface swabs are analyzed on the MicroPRO with results within 5 minutes
  • Data generated by the MicroPRO not only provides information about microbial populations but also indicates levels of residual product
environmental swab protocol specific pathogens salmonella
Environmental Swab Protocol –specific pathogens (Salmonella)
  • Place a swab sample in a 5mL snap-cap tube containing 900µL Buffered Peptone Water
  • incubate at 42oC (+2oC) with rocking* for 3 hours
  • Add 1mL 2X strength RV broth to each tube and incubate with rocking for an additional 4 hours Press the swab against the side of the tube to express extra liquid
  • After enrichment, label samples with antibody (40 minutes)
  • Dilute enriched samples in phosphate buffer
  • Analyze samples and controls on the MicroPRO for the presence/absence of Salmonella spp.
environmental swab protocol specific pathogens salmonella1
Environmental Swab Protocol –specific pathogens (Salmonella)

Positive (22 isolates tested)

Negative control

enumeration of fermentation pure cultures
Enumeration of Fermentation/Pure cultures
  • Collect sample; if necessarydilute to <106 cfu/mL in PB
  • Dispense 3mL sample into 5mL snap-cap tube
  • Load sample into RBD 3000 Sample Tray with capped syringe
  • Select pre-defined (or create new) Analysis/Tray Sequence
  • MicroPRO count result in 5 minutes
tvo escherichia coli
TVO - Escherichia coli

PB Background

~101 cfu/mL

~102 cfu/mL

~103 cfu/mL

~104 cfu/mL

Box = 1 count/0.25 mL

Box = 3 count/0.25 mL

Box = 36counts/0.25 mL

Box = 401 counts/0.25 mL

Box = 3725 counts/0.25mL

*RBD 3000 counts/mL are background corrected and have been adjusted for reagent additions.

slide59

Correlation of RBD 3000 TVO Counts vs. Plate Counts

(Poster presented at SIM 2006)

Candida albicans (n = 14, R2 = 0.9982), Escherichia coli (n = 17, R2 = 0.9959), Mycoplasma bovis (n = 15, R2 = 0.9891)and Salmonella typhimurium (n = 15, R2 = 0.9952).

slide60

Correlation of RBD 3000 TVO Counts vs. Plate Counts

(Poster presented at SIM 2006)

Bacillus atrophaeus (n = 15, R2 = 0.9839), Clostridium perfringens Type A (n = 12, R2 = 0.9981), Staphylococcus aureus (n = 18, R2 = 0.9857) and Streptococcu bovis (n = 13, R2 = 0.9832)

some of the microorganisms enumerated with the micropro
Some of the Microorganisms Enumerated with the MicroPRO
  • Enterococcus faecium
  • Enterococcus faecalis
  • Enterococcus gallinarum
  • Enterococcus hirae
  • Enterococcus mundtii
  • Erysipelothrix rhusiopathiae
  • Escherichia coli
  • Escherichia coli O157:H7
  • Escherichia coli O25:HN
  • Escherichia coli O15:NM
  • Escherichia coli O1:NM
  • Escherichia coli O7:NM
  • Escherichia coli O78:NM
  • Escherichia coli ON:H8
  • Escherichia coli ON:NM
  • Escherichia coli O8:HN
  • Geobacillus stearothermophilus
  • Geobacillus stearothermophilus spores
  • Giardia lamblia cysts
  • Haemophilus parasuis
  • Haemophilus somnus
  • Halobacterium salinarum
  • Klebsiella pneumoniae
  • Lactobacillus acidophilus
  • Lactobacillus casei
  • Lactobacillus delbrueckii
  • Lactobacillus lindneri
  • Lactobacillus plantarum
  • Lactococcus lactis
  • Lawsonia intracellularis
  • Leptospira pomona
  • Listeria grayi
  • Listeria innocua
  • Listeria ivanovii
  • Listeria monocytogenes
  • Listeria seeligeri
  • Listeria welshimeri
  • Micrococcus candicans
  • Micrococcus luteus
  • Moraxella bovis
  • Aeromonas caviae
  • Aeromonas hydrophila
  • Aspergillus niger spores
  • Bacillus atrophaeus
  • Bacillus atrophaeus spores
  • Bacillus pumilus
  • Bacillus pumilus spores
  • Bacillus subtilis
  • Bacillus subtilis spores
  • Bordetella bronchisceptica
  • Brachyspira (Serpulina) hyodysenteriae
  • Burkholderia cepacia
  • Campylobacter jejuni
  • Candida albicans
  • Candida glabrata
  • Citrobacter freundii
  • Clostridium perfringens
  • Cryptococcus spp.
  • Cryptosporidium parvum oocysts
  • Enterobacter aerogenes
  • Enterobacter cloacae
  • Enterococcus casseliflavus
  • Enterococcus durans
slide62

Some of the Microorganisms Enumerated with the MicroPRO

  • Mycoplasma bovis
  • Mycoplasma hyopneumoniae
  • Nannocystis exedens
  • Oxalobacter formigenes
  • Pantoea agglomerans
  • Pasteurella multocida
  • Pediococcus acidilactici
  • Pediococcus damnosus
  • Proteus mirabilis
  • Pseudomonas aeruginosa
  • Pseudomonas fluorescens
  • Pseudomonas putida
  • Ralstonia pickettii
  • Raoutella terrigena
  • Saccharomyces cerevisiae
  • Salmonella adelaide
  • Salmonella anatum
  • Salmonella choleraesuis
  • Salmonella dublin
  • Salmonella enteriditis
  • Salmonella hadar
  • Salmonella heidelberg
  • Salmonella iverness
  • Salmonella schalwijk
  • Salmonella typhimurium
  • Salmonella worthington
  • Serratia marcescens
  • Shigella boydii
  • Staphylococcus aureus
  • Staphylococcus epidermidis
  • Staphylococcus saprophyticus
  • Stenotrophomonas maltophila
  • Streptococcus bovis
  • Streptococcus equinus
  • Streptococcus pyogenes
slide64

Overview

  • Contamination Level Test
    • For materials that contain low-level bio-burden
    • Determines that material meets microbial specification
    • Utilizes the Most Probable Number (MPN) protocol
    • Useful for complex matrices
  • Antimicrobial Effectiveness Test
    • Rapidly screens candidate preservative systems
    • Tracks an increase in dead cell count
slide65

Contamination Level Test –

Feasible in a Variety of Complex Matrices

  • Company A – In-process pharmaceutical product
  • Company B – Various nutraceutical finished products
    • Juice, Body Butter, Fiber
  • Company C – Liquid nutraceutical finished product
  • Company D – Various alcoholic beverages
slide67

CLT – Results Company A

In-process Product: Specification <1000cfu/g

Spiked Below Specification

Product contains >100cfu/g and <1000cfu/g; product is within specification

Actual spike was 559 cfu Ps. aeruginosa/g product

Spiked Above Specification

Product contains >1000cfu/g; product fails

Actual spike was 3920 cfu Ps. aeruginosa/g product

slide68

CLT – Results Company B

Body Butter & Juice Products: Specification <100cfu/g or mL

Body Butter: Spiked Below Specification

Product contains >10cfu/g and <100cfu/g; product is within specification

Actual spike was 35 cfu C. albicans/g body butter

Juice: Spiked Above Specification

Product contains >100cfu/g; product fails

Actual spike was 110cfu C. albicans/mL juice

  • Taken fromposter presentation at ASM 2006 A Rapid Most Probable Number Test for Yeast in Nutraceutical Products, K.A. Molitor and A.M. Steger, Advanced Analytical Technologies, Inc., Ames, IA 50010 and D. Wright and M. Roblin, Morinda, Inc., Orem, UT 84604
slide69

Contamination Level Test – Benefits

  • 18-24hr time-to-result
  • Detects lower levels of potential microbial contamination than the traditional plate count method in complex/opaque matrices
  • Results are confirmed by the “Most Probable Total Count by Multiple-Tube Method” in USP Chapter <61>, Microbial Limits Test
slide71

Antimicrobial Effectiveness Test

  • Used to screen candidate preservatives for liquid products
  • Pharmaceutical and Cosmetic Industries routinely perform
    • New & reformulated products
  • Problems with the current method:
    • Compendial method takes 35 days
    • Requires significant labor & materials
    • Not all types &/or formulations of antimicrobials are screened due to time & labor constraints
    • Delays development of suitable preservative systems
    • Delays product time to market
slide72

Antimicrobial Effectiveness Test –

Solution

  • Use the RBD 3000 to rapidly screen candidate preservative systems
  • Benefits:
    • Shorter time to result
    • Significant labor and materials savings
    • Evaluate more types/formulations of antimicrobials
    • Develop better preservative systems
    • Speed product time to market
slide73

Antimicrobial Effectiveness Test –

Protocol

  • Challenge 0.01% (w/v) Benzoic Acid with 105 – 106 cfu/mL
  • Perform challenge studies with Ps. aeruginosa, S. aureus, and C. albicans
  • Use RBD 3000 to determine titer of inoculum
  • Sample at 0, 2hr, 4hr, 6hr, 8hr & 24hr post-inoculation
    • Dilute samples 1:10 in phosphate buffer for RBD 3000 Biomass & Dead Cell counts using the Biomass and Dead Cell Test Kits
    • Dilute samples in phosphate buffer + 4% Tween 20 for TSA plate count comparison
slide74

0.01% Benzoic Acid Challenged with

Ps. aeruginosa – Test 1

slide78

Summary

  • Contamination Level Test
    • Provides a rapid & sensitive detection method for determining if materials meet particular microbial specifications
  • Antimicrobial Effectiveness Test
    • Provides a rapid method for screening candidate preservative systems
advanced analytical provides solutions for

Purified Water

  • Final Product Testing/Raw Material Testing
  • Fermentation/Animal Health/Vaccines
  • Pure cultures/ Spore preparations (US Army)
  • Residue testing/swab testing
  • Specific Pathogen testing
  • Challenge Tests

Advanced Analytical Provides Solutions for

benefits of micropro
Benefits of MicroPRO
  • Détection simultanée des bactéries, levures et moisissures en 24 heures dans plus de 95% des produits testés à ce jour et pratiquement 100% en 48 heures
  • Détection simultanée des bactéries, levures et moisissures en 5 minutes dans l’eau purifiée et l’environnement
    • Cela vous permet de prendre des actions correctives immédiatement afin de ne pas contaminer tout un lot.
  • Limite de quantification égale à 20 ufc/mL
    • Soit 5 fois mieux que notre concurrent le plus proche grâce à l’utilisation d’un laser émettant dans le rouge (moins de bruit généré par les produits et l’eau à cette longueur d’onde) et à une meilleure conception de notre cellule
  • Plus grande stabilité, plus longue durée de vie de notre laser qui est en fait une diode laser émettant dans le rouge par rapport à un laser émettant dans le bleu.
  • Cellule en quartz dont la conception évite les bouchages et contamination internes
benefits of micropro1
Benefits of MicroPRO
  • La technologie de nos réactifs permet de marquer 100% des organismes morts et vivants ou vivants seulement ou mort seulement
    • La technique de marquage consiste à fixer une sonde fluorescente sur les acides nucléiques des micro-organismes. Il ne s’agit pas d’une réaction enzymatique qui est souvent incomplète ou qui ne génère pas suffisamment de fluorescence pour être détectée. Nous pouvons aussi concevoir des kits pour marquer des micro-organismes spécifiques sur demande.
  • Les réactifs peuvent se conserver pendant 10 jours à température ambiante dans les réservoirs de l’instrument
    • Par rapport aux technologies concurrentes, vous évite beaucoup de perte et donc limite les coûts. Cette technologie permet d’utiliser le système même pour un seul échantillon alors que dans la plupart des autres techniques il est préférable d’attendre d’avoir suffisamment d’échantillons à analyser avant d’ouvrir un kit qui ne se conservera que quelques heures après l’ouverture.
benefits of micropro2
Benefits of MicroPRO
  • Très grande reproductibilité
    • Le MicroPRO ajoute les réactifs lui-même contrairement à d’autres techniques qui nécessitent de les ajouter manuellement avant introduction dans l’automate. Les temps d’incubation sont donc les mêmes pour des échantillons identiques ce qui garantie une très grande reproductibilité des résultats.
  • La quantité de réactifs nécessaire à l'analyse de quantités inférieures à 100 ou 200 échantillons par semaine peut être dosée, ce qui permet de remettre le reste au frais pour un usage ultérieur plusieurs semaines ou mois après l'ouverture de l'emballage
    • Ceci est également une source d'économie considérable par rapport à d'autres techniques.
benefits of micropro3
Benefits of MicroPRO
  • La capacité du MicroPRO est de 42 échantillons sur 3 racks de 12 échantillons et 1 rack prioritaire de 6 échantillons. Lorsque le MicroPRO a terminé l’analyse d’un rack il est possible de le sortir et de le recharger sans interrompre la séquence d’analyse
    • Par conséquent le système peut fonctionner en continu toute la journée.
    • Si des échantillons sont positionnés sur le rack prioritaire, le système les détectera et interrompra la séquence d’analyse sur un des racks standard après l’analyse des échantillons en cours. Il analysera les échantillons du rack prioritaire et reviendra à l’endroit où il s’était arrêté sur le rack standard pour poursuivre la séquence.
    • Ceci vous permet d’analyser des échantillons en urgence sans devoir arrêter manuellement la séquence d’analyse.
    • Les racks n’ont pas besoin d’être totalement remplis pour démarrer une analyse. A partir du moment où un seul échantillon se trouve sur un rack, l’analyse peut démarrer.
benefits of micropro4
Benefits of MicroPRO
  • Pas de risque de contamination croisée
    • Les racks sont conçus pour recevoir 12 échantillons ou 6 échantillons pour le rack prioritaire ainsi que 12 ou 6 seringues à usage unique d’un millilitre
    • Ces seringues sont utilisées par l’automate pour injecter chaque échantillon une seule fois dans le cytomètre.
    • Cela permet d’éliminer un risque de contamination important lorsque la même seringue est utilisée pour injecter tous les échantillons.
    • Par ailleurs le port d’injection du cytomètre est lavé systématiquement et automatiquement entre deux injections avec une solution de détergent qui a été qualifiée par le fabriquant. Cette fonction n’est pas débrayable par l’utilisateur ni les managers qui entrent les méthodes dans le système. Cela garantit une absence totale de risque de contamination croisée.
    • Notre système à fait l’objet de tests très approfondis à la fois en interne et par nos clients pour nous assurer que le risque de contamination est totalement absent.
benefits of micropro5
Benefits of MicroPRO
  • Le système permet d’analyser jusqu’à 20 échantillons par heure.
  • Le logiciel très convivial répond à la norme 21 CFR Part 11 exigée par l’industrie pharmaceutique. Ce logiciel permet de visualiser les résultats en ufc/ml ou en pass/ambiguous/fail à condition d'avoir préalablement indiqué les limites pour accepter un lot, le refuser ou dans certain cas de préciser si la mesure est ambiguë et ainsi relancer des analyses complémentaires. Ce logiciel comprend plusieurs niveaux.
    • Le premier est le niveau utilisateur qui ne permet pas de modifier ou d’entrer des nouvelles méthodes.
    • Le deuxième niveau est le niveau manager qui permet d’entrer ou modifier des méthodes.
    • Le troisième niveau est le niveau administrateur
    • Le quatrième niveau service permet à nos équipes de maintenance d’intervenir sur le système en coupant toutes les sécurités.
slide87

RBD 3000/MicroPRO References

Miller, M. J., Encyclopedia of Rapid Microbiological Methods, Volume 2, DHI Publishing, River Grove, IL, USA.  2005.

Chapter 16: Steger, A. M. “Rapid enumeration of microorganisms using Advanced Analytical’s RBD 3000.”Encyclopedia of Rapid Microbiological Methods, Volume 2. Ed. M. J. Miller. River Grove, IL, USA: DHI Publishing, LLC, 2005. (AATI)

Chapter 17: Kozak, K. C. and D. E. Langworthy.  “Rapid Microbial Counting by Flow Cytometry: Validation and Implementation for Research and Development (R&D) Applications.”Encyclopedia of Rapid Microbiological Methods, Volume 2. Ed. M. J. Miller. River Grove, IL, USA: DHI Publishing, LLC, 2005. (P&G)

Chapter 18: Homesley, P. H.  “The RBD 3000 Rapid Bacterial Enumeration System as an Alternative to Traditional Pour Plate Enumeration.”Encyclopedia of Rapid Microbiological Methods, Volume 2. Ed. M. J. Miller. River Grove, IL, USA: DHI Publishing, LLC, 2005. (J&J)

for more information
For More Information

Disruptive Technologies

3 allée des Camélias

94440 Villecresnes

France

Contact: William Amoyal

Tel. +33 (0)6 98 64 98 81

Email : wamoyal@disruptechno.com

slide89

Thank You !

www.aati-us.com

www.disruptechno.com