AFFYMETRIX chips. What are DNA chips/arrays?. Small silica (glass) chips covered with thousands of nucleotides of know sequence Underlying principle: Base pairing or hybridization Medium for matching known and unknown DNA samples Two types of arrays-Macro and Micro
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Macro-Sample spot sizes >= 300 microns
Micro-Sample spot sizes < 200 microns in diameter
1) probe CDNA
2) AFFYMETRIX DNA chips
- Truncation used to prevent formation of probes with missing nucleotides
- Also, side chains of nucleotides are protected, to prevent the formation of branched oligonucleotides.
TWO MAIN CLASSES:
Colors representing gene activity (red is high, blue is low) show that MLL has a distinct pattern from both ALL and AML. Each vertical column of squares represents a tumor sample. Each horizontal row of squares represents the activity of one gene.