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微四 92134048 黃昭瑜

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微四 92134048 黃昭瑜

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  1. Development of a biofilm Production-Deficient Escherichia coli Strain as a Host for Biotechnological ApplicationsBong Hyun Sung, Choong Hoon Lee, Byung Jo Yu, Jun Hyoung Lee,Ju Young Lee, Mi Sun Kim, Frederick R. Blattner, and Sun Chang KimApplied and Environmental Microbiology. 2006. Vol. 72, No. 5 , p. 3336-3342 微四 92134048 黃昭瑜

  2. 尋找方法 • 到AEM首頁 • 進入搜尋系統 • Key word：Biotechnological 、Bacteria、industry、年份2006 • 459篇

  3. Escherichia coli形成Biofilm的3種成份： Curli ：讓細胞能附著不同表面和互相黏著 (構成基因：csgG-csgC ) Colanic acid ： Biofilm結構的主體 (構成基因：wcaL-wza ) Type I pili ： 初始附著 (構成基因：fimB-fimH )

  4. 目的 • 藉由去除以上三種成份的構成基因，檢視去除這些基因的影響性，和生技上的益處。

  5. 利用Red-mediated markerless deletion 技術去除三種基因，製造出一個不會生產生物膜的新菌株BD123

  6. Red-mediated markerless deletion • 為一種較有效率的同源重組技術 • 具有同源序列短(40～60 bp)、重组效率高的特点.這种技術可在DNA目標分子的任意位点進行基因敲除、敲入、点突變等操作,無需使用限制性内切酶和連接脢.

  7. 結果 • 製造出來的菌株BD123增加了重組蛋白的分泌度、抗生素的敏感度、轉型率增加。 • 可以用此特性使BD123作為一個良好的host用於各方面的生物技術 。

  8. Thank You

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