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Genetic engineering involves transferring genetic material between organisms, resulting in Recombinant DNA. Learn how human genes controlling insulin and growth hormone have been inserted into bacteria to synthesize necessary substances. Follow the steps, including cutting donor DNA, opening plasmids, inserting donor DNA, and bonding with ligase to create synthesized proteins.
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What is it? • Involves the transfer of genetic material from one organism to another. • Results in the formation of Recombinant DNA. • Human genes that control the synthesis of insulin and growth hormone have been inserted into bacteria. • Bacterial cells are then able to synthesize (make) certain substance needed by humans!
Steps: • Cut out donor DNA with restriction enzyme • Cut open plasmid with restriction enzyme • Insert donor DNA into open plasmid • Add ligase to bond donor DNA and plasmid • Obtain synthesized protein https://www.youtube.com/watch?v=TpmNfv1jKuA