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Morphological structure and water status in bulbs during their transition from dormancy to active growth: Visualization by NMR imaging. Rina Kamenetsky Hanita Zemah Annette van der Toorn Haim D. Rabinowitch Henk van As Peter Bendel accepted by J. exp. Bot.
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Morphological structure and water status in bulbs during their transition from dormancy to active growth: Visualization by NMR imaging Rina Kamenetsky Hanita Zemah Annette van der Toorn Haim D. Rabinowitch Henk van As Peter Bendel accepted by J. exp. Bot.
Tulip bulbs (Tulipa gesneriana L.) • World wide use of tulip as ornamental plant • Needs a cold period during dormancy to facilitate normal development and flowering • Fundamental knowledge about bulb dormancy and growth is limited
Purpose of the project: To determine if significant differences in water status between bulbs stored at 4 oC and at 20 oC can be observed using MRI. Mainly using: • Visible morphological changes in the images • Proton density determination • R2 measurements (1/T2) • ADC measurements (reflecting proton mobility) • R1 measurements (1/T1)
Morphological MR image of tulip bulb scales bud basal plate 1 cm Resolution 350 x 350 x 700 mm
Single parameter images of the tulip bulb Amp of R1 Amp of R2 Amp of ADC 0.033 0.025 0.033 0 0 0 2.5 30 2.5e-9 0 0 0 R1 image R2 image ADC image
7 20 oC 4o C 30 20 oC 4 oC amplitude Time- dependent change in R2 for two bulbs R2 Time- dependent change in amplitude for two bulbs 0 0 start 2 weeks 4 weeks 8 weeks 12weeks
Planted bulbs (10 days after planting) 4 oC 20 oC
Conclusions • MR parameters such as R2 and ADC can be used to observe differences between chilled and non-chilled bulbs • MRI needs to be combined with histology to understand the observed changes in water status • The majority of changes in water status take place between 2 and 8 weeks after storage