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ActD 0h. ActD 6h. 120.0. 100.0. 80.0. (% of ActD 0h). Reporter activity. 60.0. 40.0. 20.0. 0.0. AANAT. -. 3‘UTR. 1263. 449. 1889. 120. 100. 80. % of activity remaining. 60. 40. NAT. 3'U1889. 20. 3'U1263. 0. 0. 0.5. 1. 1.5. 2. [h]. Woo et al_.

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ActD 0h

ActD 6h

120.0

100.0

80.0

(% of ActD 0h)

Reporter activity

60.0

40.0

20.0

0.0

AANAT

-

3‘UTR

1263

449

1889

120

100

80

% of activity remaining

60

40

NAT

3'U1889

20

3'U1263

0

0

0.5

1

1.5

2

[h]

Woo et al_

mper2 3′UTR end sequence is responsible for its own degradation

Supplementary Fig. S1

A

B


+

3′U1889

3′U1877

+

IP

+

3′U1263

+

3′U1660

3′U449

+

+

+

+

3′U1440

+

PTB

+

Ab

3′U1263

#1

+

+

+

+

competitors

SCN-N

SCN-N

+

+

+

+

+

+

90.5

90.5

90.5

61.5

61.5

61.5

46.2

46.2

46.2

37.8

37.8

37.8

+

+

3′U449

#1

competitor

+

+

+

3′U1889

+

+

+

+

+

3′U449

+

S100

+

+

++

Cold-3′U449

purified-PTB4 (ng)

25

400

+

+

+

NIH3T3-c

90.5

90.5

61.5

61.5

46.2

46.2

37.8

26

Woo et al_

PTB binds to pyrimidine tract in mper2 3′end sequence in SCN

Supplementary Fig. S2

A

B

PIS

C

E

D


Cyto.

Nuc.

6

18

6

18

12

24

12

24

PTB

hnRNP D (short Exp.)

hnRNP D (long Exp.)

9

18

21

0

6

12

14

16

Cyto.

Nuc.

18

0

6

12

14

16

Lamin B

14-3-3ξ

Woo et al_

Cytoplasmic PTB differentially translocalizes at respective phase

Supplementary Fig. S3

A

B


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