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Understanding Microscopes: Parts, Functions, and Magnification Techniques

This educational content focuses on the fundamental aspects of microscopes essential for aspiring laboratory assistants. Students will learn to identify the components of a microscope, including monocular and binocular types, as well as specialized models like the epifluorescence microscope. The session includes engaging internet activities that reinforce their learning of microscope parts and magnification techniques. Additionally, it aligns with state standards for health and disease prevention education, empowering students with valuable skills.

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Understanding Microscopes: Parts, Functions, and Magnification Techniques

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  1. Students will grade Nutrition Test as a class. • TO: Identify parts of the microscope. • Students will complete A – Z Microscope Internet Activity. • Closure: You’re Stuck Here Until…. 3/5/14 Today’s Agenda: State Standards: 9.2 Describestrategies for prevention of diseases including health screenings and examinations. Unit 5: What skills are necessary to be a Laboratory Assistant?

  2. Microscopes • Invented by Antony van Leeuwenhoek in 16C who then discovered bacteria. • 2 Models - • Monocular has 1 eye piece • Binocular has 2 eyepieces • Epifluorescence microscope detects antibodies and specific organisms by using a fluorescent dye stain

  3. Monocular Binocular

  4. Parts of a Microscope • Base – solid stand • Arm – long stem, used to carry

  5. Parts of a Microscope • Eyepieces – aka Ocular viewpeice, magnifies the object usually 10x or 20x, piece that you look through

  6. Parts of a Microscope • Objectives – magnify the object being viewed • Low-power = 4x • Low-power = 10x • High-power = 40x • High-power = 45x • Oil-immersion (OI) = 95x • Oil-immersion (OI) = 100x (when image is too dark)

  7. Parts of a Microscope • Revolving Nose Piece – where objectives attach, turn to use different objective • Stage – flat platform c slide clips to hold slide in place

  8. Parts of a Microscope • Course Adjustment Knob – moves objective up and down, rough focus • Fine Adjustment Knob – moves objective slowly for clear focus

  9. Parts of a Microscope • Iris Diaphragm– controls amount of light to enter the microscope • Illuminating Light– light source • Body Tube – connect the eye piece and objectives.

  10. To Determine Magnification: • Multiply the power of the eye piece times the power of the objective in use. • Examples: • The eyepiece is 10x and the objective is 4x (10)(4) = 40 times • The eyepiece is 20x and the objective is 40x (20)(40) = 800 times

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