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This study presents a detailed analysis of Brn-3a and p73 interaction in neural cells using immunofluorescence at 60x magnification. The overlay images show the colocalization of Brn-3a and p73, alongside DNA content and cell number quantification. The data provides insights into cellular processes involving Brn-3a and p73 in neural cells.
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Supplementary Fig S1 x60 Brn-3a mAB-FITC p73-SAM pAB-Rh Brn-3a/p73 overlay hoechst Phase X 60 Phase NF-160 mAB-FITC p73-SAM pAB-Rh NF-160/p73- overlay A B
A Supplementary Fig S2 Cell number Cell number 70 (-) Brn-3a ** 60 (+) Brn-3a DNA content DNA content * 50 40 % of cells in G1 phase Cell number Cell number 30 20 DNA content DNA content 10 0 Cell number p73α p73β Cell number Vector Brn-3a ΔNp73 p73βΔOD DNA content DNA content Cell number Cell number DNA content DNA content Cell number Cell number DNA content DNA content B