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Evolution of eDNA Monitoring for Asian Carp in CAWS: Objectives & Results

Explore the journey of utilizing eDNA as a monitoring tool for bighead and silver carp in the CAWS region. Learn about the transition process, key steps, results, and future plans highlighted in the MRRWG Asian Carp eDNA monitoring project.

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Evolution of eDNA Monitoring for Asian Carp in CAWS: Objectives & Results

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  1. 2.6.1 eDNA Monitoring of the CAWS

  2. Introduction and Objectives • UND-USACE (cooperative agreement): eDNA as a monitoring tool to indicate genetic presence of bighead and silver carp DNA • July 2009 – May 2010 • Transition to USACE (independent sample, filtration, processing) • June – September 2010 • As part of MRRWG MRRP, eDNA linked to other actions • October – December 2010

  3. RCC Monitoring Battledrill Step 1 Step 3 Step 1: Illinois: Multi-Agency Sample Step 2: Illinois: USACE eDNA Filtering at EPA lab Step 3: Mississippi: Process eDNA at ERDC lab Step 4: ERDC eDNA Reports Step 5: MRRWG notified Step 6: Results posted online MRRWG eDNA sampling 2010 Strategy • 120 samples/week Priority areas (rotation): • Wilmette Station • Chicago Lock • O’Brien Lock Secondary areas (requested): • Lockport Pool (above/below Barrier) • Des Plaines River (Once/quarter) • Process • Samples collected • Samples filtered at EPA • Sample filtrate sent to ERDC overnight • Preliminary eDNA Results • Final Results • Posted to LRC website

  4. MRRWG Asian Carp eDNA Monitoring Results Above Barrier (2010) • Highlighted row shows most recent positive detection (12/7/2010) MRRWGAsian Carp eDNA Monitoring Results Below Barrier (2010) ALL 2010 SAMPLES PROCESSED AND REPORTED http://www.lrc.usace.army.mil/AsianCarp/eDNA.htm

  5. Future Plans • MRRP identifies eDNA as a useful tool to indicate genetic presence of bighead and silver carp in CAWS; • Latest draft of MRRP has eDNA as a tool to continue surveillance of AC DNA above Barriers; • Final strategy of eDNA as surveillance tool still not decided

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