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Factors Influencing Cellular Respiratory Rate and Energy Production

Explore the control of respiratory rate and factors affecting energy production in cells, such as substrate availability, oxygen levels, electron transfer rate, ADP and Pi availability, proton gradient, and determination of P/O ratios. Additionally, learn about processes influenced by proton gradient like bacterial cell transport, Ca2+ movement, flagella rotation, temperature regulation, and the role of brown fat and uncoupling protein. Optional reading on uncoupling proteins available.

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Factors Influencing Cellular Respiratory Rate and Energy Production

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  1. Control of respiratory rate • Availability of substrate (NADH or FADH2) • Availability of oxygen • Rate of electron transfer between and within electron • transport complexes • Availability of ADP and Pi • Proton gradient – DG of proton translocation

  2. pO2 TIME Determination of P/O ratio NADH generating substrate

  3. pO2 TIME Determination of P/O ratio succinate

  4. Substrate pO2 TIME

  5. Substrate pO2 TIME

  6. NADH generating substrate pO2 TIME

  7. NADH generating substrate pO2 TIME

  8. Some additional processes driven by proton gradient • sugar and amino transport in bacterial cells • Ca2+ movement into mitochondria • rotation of flagella in some bacteria • temperature regulation • brown fat, skunk cabbage, thermogenin (uncoupling protein) Optional reading The following web sites gives additional information on the family of uncoupling proteins: http://lsvl.la.asu.edu/bio569/jhazel/uncouplingproteinwebsite/Uncoupling%20Proteins%20Home.htm

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