Biology EOC Sample Questions. Follow up to: Foaming Spuds Experiment Review slides 2-5 on your own, then answer questions on slides 6-8. Experiment: Foaming Spuds Introduction:
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Biology EOC Sample Questions Follow up to: Foaming Spuds Experiment Review slides 2-5 on your own, then answer questions on slides 6-8.
Experiment: Foaming Spuds Introduction: Hydrogen peroxide in cells breaks down into water and oxygen. When this reaction happens, bubbles of oxygen gas released produce foam. Catalase, a protein found in all cells increases the rate of this reaction. Low pH level (acid) reduces the ability of catalase function. To test this idea, an experiment was performed using potato juice as the source of catalase.
Procedure: • Label four graduated cylinders with pH 6, 7, 8, 9 • Put 10 ml of potato juice at the correct pH in each of the cylinders. • 4. Be sure the room temperature remains the same throughout the investigation. • 5. Add 5 ml of hydrogen peroxide to each graduated cylinder, stir for two seconds. Wait three minutes. • 6. Measure and record the volume of foam in each graduated cylinder and record. • 7. Clean all graduated cylinders and stirring rods. • 8. Repeat steps 1 through 7 for a total of 4 trials. • 9. Calculate and record the average volume of foam for each acidity of potato juice.
Data: Directions: Use the previous information to answer the questions that follow. Be able to justify your answer choice
Now respond to these questions on your own on a separate sheet of paper. 1. What is the experimental question in this scenario?
2. Why is the data collected for the Foaming Spuds Experiment considered to be reliable? • 4 different pH levels were tested • Potato juice was used a source of “natural” catalase • The experiment included multiple trials • Foam was measured as an indicator of oxygen production
3. Use the information on slide 5 to write a conclusion to the Foaming Spuds Experiment In your conclusion, be sure to: • Answer the experimental question. • Include supporting data from the Volume of Foam vs Ph Level graph. • Explain how the data supports your conclusion. • Give a scientific explanation for the trend in the data.