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pCDB 3

A). B). T7. liuB. liuD. pH. 6-His. pCDB 3. S-Tag. 7285 bp. T7. Sm. lacI. Temperature ( o C).

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pCDB 3

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  1. A) B) T7 liuB liuD pH 6-His pCDB 3 S-Tag 7285 bp T7 Sm lacI Temperature (oC) Figure S1.- A) Co-expression plasmid pCDB3. The plasmid contains both subunits of the MCCase from P. aeruginosa under the promoter T7 control. The α-subunit and β-subunit encoded by liuD and liuB genes were fusioned with 6-His and S-Tag epitopes, respectively. B) pH and temperature behavior for the recombinant MCCase enzyme.

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