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Cellular Association of E2Fs with p130 Mutants in CHO Cells

This study investigates the interaction between E2F transcription factors and wildtype (wt) and mutant p130 (C894F) in CHO cells. The indicated constructs were transfected into CHO cells, and cell extracts harvested two days later. Immunoprecipitation was performed using specific E2F antibodies, followed by western blot analysis with anti-HA and anti-E2F antibodies.

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Cellular Association of E2Fs with p130 Mutants in CHO Cells

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  1. A. E2F1 + + + HA-p130 + HA-p130C894F + E2F4 + + + HA-p130 + HA-p130C894F + E2F5 + + + HA-p130 + HA-p130C894F + IB: HA IB: HA IB: HA IB: E2F5 IB: E2F1 IB: E2F4 1 2 3 4 5 6 7 8 9 IP: E2F1 IP: E2F4 IP: E2F5 C. B. Fig S4 Cellular association between E2Fs and wt and mutant p130(C894F). (A-C) The indicated constructs were transfected into CHO cells and cell extracts harvested two days later. The extracts were subjected to immunoprecipitation with the indicated E2F antibodies and immunoprecipitates were analyzed by western blotting using anti-HA or anti-E2F antibodies as indicated.

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