IP: E2F1 - PowerPoint PPT Presentation

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  1. A. E2F1 + + + HA-p130 + HA-p130C894F + E2F4 + + + HA-p130 + HA-p130C894F + E2F5 + + + HA-p130 + HA-p130C894F + IB: HA IB: HA IB: HA IB: E2F5 IB: E2F1 IB: E2F4 1 2 3 4 5 6 7 8 9 IP: E2F1 IP: E2F4 IP: E2F5 C. B. Fig S4 Cellular association between E2Fs and wt and mutant p130(C894F). (A-C) The indicated constructs were transfected into CHO cells and cell extracts harvested two days later. The extracts were subjected to immunoprecipitation with the indicated E2F antibodies and immunoprecipitates were analyzed by western blotting using anti-HA or anti-E2F antibodies as indicated.