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Electron Microscopes. Use electrons instead of light to form images. Light Microscopes. Light Microscopes. Unknown when first invented, but probably between 1590-1610 A.D. Can magnify up to 1000 x

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electron microscopes

Electron Microscopes

Use electrons instead of light to form images.

light microscopes1
Light Microscopes
  • Unknown when first invented, but probably between 1590-1610 A.D.
  • Can magnify up to 1000 x
  • Shines light through an object and projects the image through a series of lenses that magnify the image.
  • Relatively inexpensive
  • Great for studying live microscopic organisms.
  • Great for studying cells and tissues in general.
  • Widely used in hospitals and clinics to diagnose disease.
transmission electron microscope tem1
Transmission Electron Microscope(TEM)
  • First invented in 1931 (in Germany)
  • Can magnify up to 200,000 x (and beyond)
  • Similar to a light microscope except that a beam of electrons is used instead of light. The electrons that penetrate through the object and create an image which is then enlarged and then viewed on a computer monitor.
  • Samples of the object must be sliced very thin before using the TEM. (No living specimens.)
  • Very expensive and time consuming to prepare slides.
uses of the tem
Uses of the TEM
  • Used in biological/medical research to investigate parts of cells and molecules.
  • Used in material science to study the structure and the weaknesses of crystals.
  • Used in nanotechnology.
scanning electron microscope sem
Scanning Electron Microscope(SEM)
  • http://www.mos.org/sln/SEM/
scanning electron microscope sem1
Scanning Electron Microscope(SEM)
  • First invented in 1942
  • Can magnify typically up to 10,000 x
  • Gives a 3D image of the surface of the object
  • Again, a beam of electrons is used instead of light. The electrons that bounce off the object create the image. The microscope scans a certain region of the object multiple times and then combines multiple images to create what we then see on a computer monitor as one 3D image.
  • No slicing needed so easier to prepare samples. Larger objects can be viewed.
  • Very expensive.
uses of the sem
Uses of the SEM
  • Multiple uses - for viewing the surface of any microscopic object.
  • Insect parts
  • Bacteria
  • Food industry
  • Material science
  • Education
  • Art
practice quiz
Practice Quiz
  • Now look at the following images. Determine which type of microscope produced each image and write your answers down on a piece of paper.
  • LM = Light Microscope
  • TEM = Transmission Electron Microscope
  • SEM = Scanning Electron Microscope