Benefits and Features of Ampholyte

2. Benefits and Features of Ampholyte Carrier Ampholyte is low molecular weight molecules of zwitterionic character. They are a mixture of falsely derived species of standard molecular weight distribution of four hundred to one thousand dalton as well as comprise a multitude of varying pI-values. In agarose as well as polyacrylamide gels containing ampholytes, a linear pH gradient will be built up when an electric field is applied - the ampholyte molecules a net charge as well as thus migrate in the electric field between the electrodes as long as they will attain the position of corresponding pI. They will impede moving then and form small plateaus (stationary stacks). Benefits are: High resolution due to multimeric composition Fast staining and destaining times

3. clear background associated with very low unspecific binding of dyes and stains high solubility in trichloroacetic acid (fast removal of ampholytes during fixation) virtually no interaction with metal ions Sample preparation is often essential to separate as well as concentrate diverse compounds prior to analysis of complex samples. In this stare, isoelectric focusing (IEF) is one of the finest sample preparation procedures. With this approach, however, carrier ampholyte has to be introduced into the samples, which may consequence in matrix interferences.