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Laboratory Methods in Biotechnology. Theory and practice of common biological laboratory methods: solution preparation, spectrophotometry, electrophoresis, DNA manipulations, and biological, chemical, and radiological safety. Idea Behind the Course.

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Laboratory methods in biotechnology

Laboratory Methods in Biotechnology

Theory and practice of common biological laboratory methods: solution preparation, spectrophotometry, electrophoresis, DNA manipulations, and biological, chemical, and radiological safety


Idea behind the course
Idea Behind the Course

  • Provide practical experiences in laboratory activities that will help students make the transition from content-based lower division courses to application orientated senior level courses.

  • Provide skills and dispositions needed to carry out laboratory research as part of a career in the life sciences. This includes undergraduate research, working as a technician, graduate research, and affiliated professions.


Role in your curriculum
Role in Your Curriculum

Core: Reasoning, Investigation,

Cell Biology, Genetics,

MCMB Specialty: Lab Methods, Micro,

Molecular Genetics

Electives: Molecular Techniques, Cell

and Molec, Immunology, Path Micro,

Microbial Phys, etc


Objectives
Objectives

  • To give students experience in dimensional analysis used in microbiology and cellular and molecular biology, and biotechnology


Objectives1
Objectives

  • To familiarize the students with the theory and practice of preparing solutions from various starting materials.


Objectives2
Objectives

  • To introduce the students to means of preparing sterile media and solutions.


Objectives3
Objectives

  • To allow students to practice quantifying bacteria and viruses, and to measure the growth rates of bacterial populations.


Objectives4
Objectives

  • To allow students to quantify proteins, nucleic acids and other chemicals in solution.


Objectives5
Objectives

  • To introduce students to commonly used devices including, but not limited to: microliter pipetters, centrifuges, spectrophotometers, pH meters, etc.


Objectives6
Objectives

  • To introduce the students to the use of enzymes for end-point determination and to the measurement of enzyme activity.


Objectives7
Objectives

  • To give students experience in the techniques for the manipulation and electrophoretic separation of proteins and DNA.


Objectives8
Objectives

  • To provide information regarding biological, chemical, radiation, and other aspects of lab safety.


Textbooks
Textbooks

  • Make sure you bring this each time.

    • Seidman and Moore – our main text

    • Leboffe and Pierce – illustration of techniques

  • Also need:

    • Calculator!

    • Lab Notebook


Additional resources
Additional Resources

  • Brock – background information on theory

  • Day – advice on writing (humor)

  • Robyt - Source of many handouts/additional readings

  • CBE Manual – Style guide for preparation of manuscripts

  • Don’t buy these


Webpage http cstl csm semo edu champine bi245 index html
Webpagehttp://cstl-csm.semo.edu/champine/BI245/index.html

  • Lab Activities

  • Lecture Topics

  • Homework Assignments

  • In Class Assignments

  • Manuscripts

  • Notebook Guidelines

  • Syllabus

  • Grade Book


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