Engineering a tmj disc
Download
1 / 15

Engineering a TMJ Disc - PowerPoint PPT Presentation


  • 191 Views
  • Updated On :

Engineering a TMJ Disc. Danielle Lewis Louisiana Tech University REU, University of Louisiana at Lafayette Dr. David Mills Louisiana Tech University. What is a TMJ Disc?. Temporomandibular Joint Disc Located between the base of the skull and the lower jaw

loader
I am the owner, or an agent authorized to act on behalf of the owner, of the copyrighted work described.
capcha
Download Presentation

PowerPoint Slideshow about 'Engineering a TMJ Disc' - benjamin


An Image/Link below is provided (as is) to download presentation

Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author.While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server.


- - - - - - - - - - - - - - - - - - - - - - - - - - E N D - - - - - - - - - - - - - - - - - - - - - - - - - -
Presentation Transcript
Engineering a tmj disc l.jpg

Engineering a TMJ Disc

Danielle Lewis

Louisiana Tech University REU, University of Louisiana at Lafayette

Dr. David Mills

Louisiana Tech University


What is a tmj disc l.jpg
What is a TMJ Disc?

  • Temporomandibular Joint Disc

  • Located between the base of the skull and the lower jaw

  • Allows for smooth opening and closing of the jaw


Tmds temporomandibular disorders l.jpg
TMDs: Temporomandibular Disorders

  • Improper disc placement hampers proper jaw movement, called disc displacements

  • 7-28% of adult population affected, mainly females between 18 and 25

  • No known treatment for disorder

  • In severe cases discs are completely removed and patients can no longer move jaw



Current obstacles in engineering a tmj disc l.jpg
Current Obstacles in Engineering a TMJ disc

  • Recreating the intricate cellular structure

  • 3 regions: anterior band, intermediate zone, and posterior band

  • Anterior and posterior bands: interlaced collagen fiber bundles

  • Intermediate zone: aligned collagen fibers along with tiny fibrochondrocytes and small elastin fibers


Approach of the mills lab electrospun scaffolds l.jpg
Approach of the Mills Lab:Electrospun Scaffolds

  • Electrospinning:

    • A high voltage is passed through a polymer solution inducing an electrostatic repulsion force

    • The polymer is pumped through an insulin syringe, the repulsion force results in the formation of a thin jet

    • This jet is directed toward a grounded collection plate, the solvent evaporates before hitting the collection plate and results in the formation of a polymer scaffold


My research plan l.jpg
My Research Plan

  • Culture bovine fibrochondrocytes (FBCs) in 3 different gel environments – agarose, collagen, alginate

  • Treat cells with growth factors to observe their effect on proliferation, cell survival and protein expression

    • bFGF

    • TGF alpha and beta

    • CTGF

  • Characterize the extracellular matrix being produced by the FBCs


Cell isolations l.jpg
Cell Isolations

  • Bovine FBCs were isolated from TMJ discs taken from cow skulls

  • Cells used in experiments were passage 6, slightly old but still exhibited the characteristic FBC shape


Gels collagen and agarose l.jpg
Gels:Collagen and Agarose

  • FBCs were suspended in both collagen and agarose gels and allowed to grow for several days, 18 day group and 8 day group


Fixing dehydration and paraffin imbedding l.jpg
Fixing, Dehydration, and Paraffin Imbedding

  • Gels fixed in 2% paraformaldehyde

  • Gels were dehydrated by exposing them to a variety of ethanol solutions then infiltrated with pariffin to preserve them indefinitely

  • Gels were then imbedded in a paraffin block in preparation for creating slides

  • Next… Immunohistochemistry


Results l.jpg
Results

  • Simple examination under phase contrast showed that FBCs thrived better in collagen gels, cells attached and displayed characteristic shape


Is there any explanation for this observation l.jpg
Is there any explanation for this observation?

  • Expected result

  • Collagen type I abundant in TMJ disc


Conclusions l.jpg
Conclusions

  • Bovine fibrochondrocytes appeared to prefer the collagen gel environment over the agarose gel environment


Next steps l.jpg
Next Steps….

  • Begin staining gels for extra-cellular matrix proteins

    • Hypothesis: FBCs grown in collagen gels will exhibit an increase in extra-cellular matrix proteins over those grown in agarose gels

  • Use this experiment as a control and continue by adding growth factors to FBCs in gel culture

  • Compare the amounts and types of extra-cellular matrix proteins found in gels, both with growth factors and without, to that found in actual disc and FBCs grown on electrospun scaffolds


Thank you l.jpg
Thank you…

  • Dr. Mills, Kanthi, Skylar, Deepak, Stephanie, Paul

  • Dr. Jones

  • Louisiana Tech for lab facilities in Carson Taylor and the BME building

  • National Science Foundation


ad