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Instrumentation and control. Parameters: to be monitored and controlled Temperature Pressure Agitator shaft power Flowrate Liquid level Viscosity Turbidity pH Redox potential Ion concentration DO Read pages 308-310 in the text book. Summary of Bioreactor Design and Operation.

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instrumentation and control
Instrumentation and control
  • Parameters: to be monitored and controlled
  • Temperature
  • Pressure
  • Agitator shaft power
  • Flowrate
  • Liquid level
  • Viscosity
  • Turbidity
  • pH
  • Redox potential
  • Ion concentration
  • DO

Read pages 308-310 in the text book.

summary of bioreactor design and operation
Summary of Bioreactor Design and Operation
  • Modified batch and continuous reactors

- Chemostat with cell recycle

Keep high cell mass concentration in the reactor.

Production of biomass and low-value product

- Fed-batch

Maintain low substrate concentration

Secondary metabolites, prevent catabolite repression

- Multi-stage chemostat reactor

Separate cell growth and product formation

Secondary metabolites, genetically modified cell culture

slide3
Summary of Bioreactor Design and Operation
  • Modified batch and continuous reactors

- Chemostat with cell recycle

(qp=0, kd ≈0, X0=0,Monod equation is applied):

slide4
Summary of Bioreactor Design and Operation
  • Modified batch and continuous reactors

- Fed-batch

(qp=0, kd ≈0, Monod equation is applied):

slide5
Summary of Bioreactor Design and Operation
  • Modified batch and continuous reactors

- Multi-stage chemostat reactor

slide6
Summary of Bioreactor Design and Operation
  • Immobilized cell system

Advantages and disadvantages

  • Operation consideration

agitation and aeration, determination of volumetric mass transfer coefficient kLa,

heat removal, foam, etc.

slide7
Summary of Bioreactor Design and Operation
  • Scale up/down: geometric and dynamic similarity:

In scale-up/down of a stirred-tank reactor, the design calculations are as follows:

  • Determine the scale-up/down factor Dp/Dm
  • Calculate the dimensions of the prototype (height H and diameter Dt of tanks, impeller diameter Di) by multiplying that of the model with the scale-up/down factor.

- Select criterion related to dynamic properties and keep it constant in both the model and the prototype.

- Determine the parameters such as impeller speed for the scale-up/down reactor.

slide8
Summary of Bioreactor Design and Operation
  • Sterilization

liquid: thermal inactivating

1- P0(t)= 1-[1-e-kdt]N0

kd = αe-E0d/RT

From the above equation:

  • Known N0, T, t, determine Kd, the probability of an unsuccessful sterilization is determined.
  • Given N0, T, acceptable probability of failure e.g. 10-3, required time can be determined
  • Higher Kd tends to achieve low probability of sterilization failure. Normally at 121oC.

Kd of vegetative cells > 1010 min-1, spores 0.5-5 min-1. The major concern is spores.

slide9
Summary of Bioreactor Design and Operation
  • Sterilization

Degradation of important compounds in the medium by thermal inactivating

ln C/C0=-kdt

where C and C0 are concentrations of the component at time t and t=0, respectively.

kd is the degradation rate constant.

kd = αe-E0d/RT

To determine the components remaining active:

the temperature T → determine kd → with known t, determine C.

slide10
Summary of Bioreactor Design and Operation
  • Bioreactor Instrumentation and control
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