MHC Restriction and IR genes. January 15, 2007. 1-1. McDevitt, H.O. and Chinitz, A. Genetic control of the antibody response: Relationship between immune response and histocompatibility (H-2) type. Science 163:1207-1208. (1969). Sandylop. Himalayan. It all began with some rabbits.
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January 15, 2007
1-1. McDevitt, H.O. and Chinitz, A. Genetic control of the antibody response: Relationship between immune response and histocompatibility (H-2) type. Science 163:1207-1208. (1969).
Synthetic polypeptide antigens
-branched multichain synthetic polypeptides
-not defined structures
-(T,G)-A-L; (H,G)-A-L; (P,G)-A-L
R=poly (tyrosine,glutamine acid
=poly (histidine,glutamic acid)
=poly (proline,glutamic acid)
Michael Sela-Weissman Institute for Sciences, Rehovot, Israel
1948- George Snell (The Jackson Laboratory)
Identified 2 types of loci:
Major (strong) = H-2
Minor (weak) = non-H2
i.e. H-1, H-4, H-Y
“Speed congenics” decrease the number of
backcross generations by typing each chromosome for background strain alleles and selecting mice to breed which have the highest number of background genes.
Inbred stains of guinea pigs
-strain 2 and strain 13
-differ at the I region of MHC
Benacerraf (1963) - Guinea Pigs
Strain 2 Strain 13
PLL(poly L-lysine) + –
tyrosine) – +
1-2. Zinkernagel, R.M. and Doherty, P.C.Immunological surveillance against altered self components by sensitised T lymphocytes in lymphocytic choriomeningitis. Nature 251:547-548. (1974).
1-3. Babbitt, B.P., Allen, P.M., Matsueda, G., Haber, E., and Unanue, E.R. Binding of immunogenic peptides to Ia histocompatibility molecules. Nature 317:359-361. (1985).
A single HEL tryptic fragment stimulates HEL specific T cells
Stimulatory fragment identified as 46-61
Allen, Strydom, and Unanue, PNAS 1984