tweak your technique electrophysiology 101 n.
Download
Skip this Video
Loading SlideShow in 5 Seconds..
Tweak Your Technique Electrophysiology 101 PowerPoint Presentation
Download Presentation
Tweak Your Technique Electrophysiology 101

Loading in 2 Seconds...

play fullscreen
1 / 28

Tweak Your Technique Electrophysiology 101 - PowerPoint PPT Presentation


  • 186 Views
  • Uploaded on

Tweak Your Technique Electrophysiology 101. August 19, 2011 Kevin Lee. What can be studied with electrophysiology?. Single channel behaviour in expression systems: HEK293, CHO’s, X. Laevis oocytes Intrinsic properties of excitable cells neurons, myocytes Synaptic transmission

loader
I am the owner, or an agent authorized to act on behalf of the owner, of the copyrighted work described.
capcha
Download Presentation

PowerPoint Slideshow about 'Tweak Your Technique Electrophysiology 101' - bat


Download Now An Image/Link below is provided (as is) to download presentation

Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author.While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server.


- - - - - - - - - - - - - - - - - - - - - - - - - - E N D - - - - - - - - - - - - - - - - - - - - - - - - - -
Presentation Transcript
what can be studied with electrophysiology
What can be studied with electrophysiology?
  • Single channel behaviour
    • in expression systems: HEK293, CHO’s, X. Laevisoocytes
  • Intrinsic properties of excitable cells
    • neurons, myocytes
  • Synaptic transmission
    • neuromuscular junction, neuronal synapses
slide12

mEPSCs (minis)

mEPSC amplitude reflects synaptic AMPAR content

Beique et al. (PNAS, 2006)

slide13

mEPSCs (minis)

mEPSC frequency can reflect:

- total number of synapses

- presynaptic function

Beique et al. (PNAS, 2006)

release probability pr of glutamate
Release probability (Pr) of glutamate

Changes in frequency of mEPSCs is generally believed to reflect changes in Pr.

“Paired-Pulse” electrical stimulation can also provide information about Pr.

Beique et al. (PNAS, 2006)

slide16

Biolistic transfection of cortical neurons in organotypic slices

Beique & Andrade (J. Physiol, 2002; cover photo)

slide18

Biolistic transfection of cortical neurons in organotypic slices

Cull-Candy et al. (Science STKE, 2004)

Barria & Malinow (Neuron, 2002)

slide19

Drawbacks to electrical stimulation:

  • Difficult to isolate individual synaptic events
  • Spatial Parameters left unknown
slide21

2P-Uncaging of MNI-Glutamate

Uncaging of MNI-Glu activates both AMPA and NMDA receptors

High spatial resolution of glutamate uncaging

Béïque et al., PNAS 2006

single synapse ca 2 imaging
Single Synapse Ca2+ Imaging

Simultaneous Glutamate Uncaging and Calcium Imaging

Uncage

1

2

Time 

1

2

fundamental dendritic properties probed by glutamate uncaging and calcium imaging
Fundamental dendritic properties probed by glutamate uncaging and calcium imaging

Losonczy & Magee (Neuron, 2006)

fundamental dendritic properties probed by glutamate uncaging and calcium imaging1
Fundamental dendritic properties probed by glutamate uncaging and calcium imaging

Losonczy & Magee (Neuron, 2006)

experimental wizardry to envy
Experimental Wizardry to Envy

Kravitz et al. (Nature, 2009)

experimental wizardry to envy1
Experimental Wizardry to Envy

Kravitz et al. (Nature, 2009)

slide28

The

End