John A.G. Briggs
Download
1 / 45

John A.G. Briggs Retrovirus structure and assembly - PowerPoint PPT Presentation


  • 153 Views
  • Uploaded on

John A.G. Briggs Retrovirus structure and assembly. The structural genomics of HIV. Gag. Immature virion. Immature HIV. p17. p24. p7. p2. p1. Gag cleavage – can be inhibited. maturation. MMTV. Immature virion. HIV. 2 pathways of Retrovirus formation. Immature virion.

loader
I am the owner, or an agent authorized to act on behalf of the owner, of the copyrighted work described.
capcha
Download Presentation

PowerPoint Slideshow about 'John A.G. Briggs Retrovirus structure and assembly' - barr


An Image/Link below is provided (as is) to download presentation

Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author.While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server.


- - - - - - - - - - - - - - - - - - - - - - - - - - E N D - - - - - - - - - - - - - - - - - - - - - - - - - -
Presentation Transcript
John a g briggs retrovirus structure and assembly

John A.G. Briggs

Retrovirus structure and assembly






John a g briggs retrovirus structure and assembly

p17

p24

p7

p2

p1

Gag cleavage – can be inhibited



John a g briggs retrovirus structure and assembly

MMTV

Immature virion

HIV

2 pathways of Retrovirus formation.


John a g briggs retrovirus structure and assembly

Immature virion

Internal scaffold domain

Cytoplasmic retention motif


John a g briggs retrovirus structure and assembly

MMTV

Immature virion

HIV

Envelopment?






John a g briggs retrovirus structure and assembly

Core formed from ~1200 capsid proteins

Arranged in ~96Å hexagonal lattice



John a g briggs retrovirus structure and assembly

Immature HIV-1

In-vitro assembled


John a g briggs retrovirus structure and assembly

If it is made from 80A hexagons, with six proteins per hexagon then 3400 ±200 gag proteins


John a g briggs retrovirus structure and assembly

Scanning Transmission Electron Microscopy hexagon then 3400

Mean mass: 165 ±29MDa

3800 ± 700 gag proteins


John a g briggs retrovirus structure and assembly

145nm immature virion formed from ~5000 Gag proteins hexagon then 3400

Arranged in ~80 Å hexagonal lattice


John a g briggs retrovirus structure and assembly

Immature virion hexagon then 3400

Tighter hexagonal lattice with 8 nm unit cell.

~5000 gag proteins form a complete shell for a 145 nm virus

Mature virion

Looser hexagonal lattice with 9.6 nm unit cell.

~1200 CA proteins form the conical core, leaving a large number elsewhere


John a g briggs retrovirus structure and assembly

Virology hexagon then 3400

University of Heidelberg

University of Oxford

Cornell University

Hans-Georg Kraüsslich

Barbara Müller

Marko Lampe

Bärbel Glass

Marc Johnson

Volker Vogt

Quentin Sattentau

Electron Microscopy

University of Oxford

Max-Planck Institute for Biochemistry

Stephen Fuller

Kay Grünewald

Friedrich Förster

Thomas Wilk

Giulia Zanetti