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Inducing Mutations in Paramecia with Ultraviolet Light. Megamecia. Introduction. Model Organism Paramecium Tetraurelia Mutagen Ultraviolet Radiation. Paramecium Tetraurelia. Ultraviolet Radiation. Electromagnetic Spectrum 100 nm – 400 nm range

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Presentation Transcript
introduction
Introduction
  • Model Organism
    • Paramecium Tetraurelia
  • Mutagen
    • Ultraviolet Radiation
ultraviolet radiation
Ultraviolet Radiation
  • Electromagnetic Spectrum
    • 100 nm – 400 nm range
  • Causes pyrimadine dimers to form in DNA (Goodsell, 2001)
    • Pyrimadine dimers may result in the formation of a point mutation.

http://www.uvsciences.com/uvtech.html

pyrimadine dimers
Pyrimadine Dimers
  • Covalent bond between two adjacent pyrimadine bases (Thyamine, Cytosine)
    • Point Mutation – Transition Mutation
      • Switching of complementary base pairs A-T becomes G-C (Russell, 2010)

http://www.mun.ca/biology/scarr/F16-20,25.jpg

http://www.uvsciences.com/images/dna.jpg

methods
Methods
  • 500 μL culture samples added to tissue culture tray
  • Samples exposed to UV radiation in a UV chamber.
    • Exposed between 10 and 60 minutes in 10 minute intervals
methods1
Methods
  • Culture trays wrapped in tin foil immediately after exposure.
    • Paramecia have the ability to repair DNA while exposed to white light
  • Repeated 30 minute exposure with new samples
    • 200 μL klebsiella inoculated wheat media added to each sample after exposure
  • Samples checked periodically for changes
    • Photos taken using a digitial microscope and Applied Vision
    • Measurements done with Image J
results
RESULTS
  • 30 minutes was the optimum time to keep the cells in UV light
  • Mutant cells were 22% larger in length & 15% larger in width
results1
RESULTS

MUTANTS

CONTROL

discussion
DISCUSSION

Other mutations that weren’t able to be cultured

discussion1
DISCUSSION
  • Experiment could be taken further
    • Analyze DNA to pinpoint where mutation occurred
  • Experimental errors
    • Tissue culture trays should have been checked for cells before exposure to Ultraviolet radiation
  • Ultraviolet radiation successfully caused a mutation
references
REFERENCES

Goodsell, D.S. (2001). The Molecular Perspective: Ultraviolet Light and Pyrimidine Dimers. The Oncologist, 6(3), 298-299. Retrieved from http://theoncologist.alphamedpress.org/cgi/content/full/6/3/298

Russell, P.J. (2010) iGenetics: A Molecular Approach 3rd Ed. San Francisco, CA: Pearson Education, Inc.