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Odile Huchette, research engineer Christèle Bellamy, research technician

G&H October 2002, Montpellier WP3 / cultivation, P4 P4 : persons involved. Odile Huchette, research engineer Christèle Bellamy, research technician Didier Perrin, research assistant Rémi Kahane, "co-ordinator ". COOPD'OR R&D-CIRAD FLHOR.

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Odile Huchette, research engineer Christèle Bellamy, research technician

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  1. G&H October 2002, MontpellierWP3 / cultivation, P4 P4 : persons involved • Odile Huchette, research engineer • Christèle Bellamy, research technician • Didier Perrin, research assistant • Rémi Kahane, "co-ordinator" COOPD'OR R&D-CIRAD FLHOR

  2. Jan. Feb. March April May June July Aug. Sept. Oct. Nov. Dec. G&H October 2002, MontpellierWP3 / cultivation, P4 Workplan 2002 • Milestones3rd year : • Differential display of clones exposed in vitro to various mineral nutrition treatments • Greenhouse experiment in various S regimes • Sampling of fresh material during the cultivation treatment for CSO analysis (P4 & P5) VITRO Micropropagation 2nd Micropropagation Experiment with various S of 4 genotypes nutrition Experiment with V. Chovelon / L. Fereol : Effect of different factors (light source, photoperiod and medium) on in vitro bulblet production (3 varieties) Green-house Greenhouse experiment COOPD'OR R&D

  3. G&H October 2002, MontpellierWP3 / cultivation, P4 Greenhouse experiment 2002  3 varieties : Printanor (PRI), Morasol (MOR), Messidrôme (MES)  3 Sulphur nutritiontreatments: 0 (in the macro-elements) = Treatment S- 2 meq (normal) = Treatment TS 8 meq = Treament S++ COOPD'OR R&D

  4. G&H October 2002, MontpellierWP3 / cultivation, P4 Greenhouse experiment 2002 01/03 : Plantation of the cloves 26/03 : first mineral nutrition (4-5 leaves) 17/06 : Harvest of Messidrôme 10-11/07 : Harvest of Morasol 16-17/07 : Harvest of Printanor Plantation in boxes with washed river sand Mineral nutrition with drop irrigation COOPD'OR R&D

  5. G&H October 2002, MontpellierWP3 / cultivation, P4 Greenhouse experiment 2002  Weekly observations : Growing of the leaves and bulbs  Measurements at harvest : -weight of the bulbs - alliin content (P5) - total sulfur (P2) in progress COOPD'OR R&D

  6. G&H October 2002, MontpellierWP3 / cultivation, P4 Growing of the 3 varieties Bulb growing = diameter of the bulb / diameter of the neck COOPD'OR R&D

  7. G&H October 2002, MontpellierWP3 / cultivation, P4 Weight of the bulbs COOPD'OR R&D

  8. G&H October 2002, MontpellierWP3 / cultivation, P4 Alliin content of the bulbs (P5) COOPD'OR R&D

  9. S- TS S++ 127 127 127 108 108 108 ALLIIN (nmol / mg fresh matter) ALLIIN (nmol / mg fresh matter) ALLIIN (nmol / mg fresh matter) 89 89 89 70 70 70 51 51 51 MES MOR PRI MES MOR PRI MES MOR PRI VARIETY VARIETY VARIETY G&H October 2002, MontpellierWP3 / cultivation, P4 Statistical analysis  influence of the variety Analysis of Variance COOPD'OR R&D

  10. MES MOR PRI 127 127 127 108 108 108 ALLIIN (nmol / mg fresh matter) ALLIIN (nmol / mg fresh matter) ALLIIN (nmol / mg fresh matter) 89 89 89 70 70 70 51 51 51 S++ S- TS S++ S- TS S++ S- TS TREATMENT TREATMENT TREATMENT G&H October 2002, MontpellierWP3 / cultivation, P4 Statistical analysis  influence of the S treatment Analysis of Variance COOPD'OR R&D

  11. G&H October 2002, MontpellierWP3 / cultivation, P4 Milestones Year 3 • Differential display of clones exposed in vitro to various mineral nutritions in progress • Greenhouse experiment in various S regimes  • Sampling of fresh material during the cultivation treatment for CSO analysis (P4 & P5)  COOPD'OR R&D

  12. G&H October 2002, MontpellierWP3 / cultivation, P4 Future experiments • In Vitro : Differential display of clones exposed in vitro to various mineral nutritions • Effect of an absence of S in the macro-elements : role of S atmospheric ? • Effect of high values of S in the medium : phytotoxicity? • Interaction between S and light (quality + quantity) • Interaction between S and N, or S and Se ? COOPD'OR R&D

  13. G&H October 2002, MontpellierWP3 / cultivation, P4 Future experiments and Milestones Year 4 • In Greenhouse (2003) : • Greenhouse cultivation of garlic bulbs enriched in CSO ? • Comparison between extreme genotypes low and high in S content and comparison virus-free and virus-contaminated garlic for sulphur composition CSO content ? •  Greenhouse cultivation of garlic bulbs providing from the experiment in greenhouse 2002 • Chemical analysis (P5) before and after processing into powder. Transfer to the health partners ? • At high level of S, effect of the N level (3 mineral nutrition treatments) ? COOPD'OR R&D

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