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This study examines the rapid analysis of common analgesics, including Acetamidophenol and Acetylsalicylic Acid, utilizing advanced HPLC techniques with SB-C18 columns and optimized particle sizes. Results show a significant decrease in analysis time, achieving complete separation of seven analgesics in under 30 seconds, while maintaining comparable resolution and sensitivity. The mobile phase includes formic acid and acetonitrile, with innovative conditions enabling high throughput and reduced solvent consumption. This method is promising for pharmaceutical applications demanding efficiency and precision.
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1 4 mAU 175 2 6 5 150 3 7 1 4-Acetamidophenol 2 Caffeine 3 2-Acetamidophenol 4 Acetanilide 5 Acetylsalicylic Acid 6 Phenacetin 7 Salicylic Acid 125 100 75 50 25 0 0 0.25 0.5 0.75 1 1.25 1.5 1.75 2 min mAU 1 175 4 150 2 6 125 5 7 100 3 3.5 um 75 50 25 LC Conditions Column: SB-C18, 4.6 x 30 mm Detector: 254 nm Injector: 1 ul, Mobile Phase: 1% Formic Acid Acetonitrile (82:18) Flow: 2.0 ml/min 0 0 0.25 0.5 0.75 1 1.25 1.5 1.75 2 min mAU 1 4 175 2 6 150 5 3 7 5 um 125 100 75 50 25 0 0 0.25 0.5 0.75 1 1.25 1.5 1.75 2 min Aumento della Risoluzione in funzione del Particle Size 1.8 um
Piccola quantità di particelle più grandi Meno particelle più piccole
0 1 2 3 4 5 6 7 min Aumento della Risoluzione in funzione del Particle Size 5µm: Rs(4,5) = 3.63 Peak Width, 5s (9) = 0.103 + 50% Resolution (Theory: + 67%) + 125% Efficiency (Theory: + 177%) 1.8µm: Rs(4,5) = 5.45 Peak Width 5s(9) = 0.075 Rs = +50% Colonna SB-C18 RRHT 4.6 x150mm Massima lunghezza
1 2 3 Rs(1,2) = 3.1 4 0.5 1 1.5 2 2.5 1100 HT + 1.8um RRHT : • Reduction of analysis time from 30 min to 2 min • Speed: 14x faster than 4.6 x 250mm, 5um • Time saving: 92% • Resolution: -35% AUMENTO DELLA VELOCITA’ D’ANALISI CON HPLC 1100 1 2 3 4.6 x 250 mm, 5 mm 1 mL/min Rs(1,2) = 4.8 29.65 N=21848 4 N=22680 0 5 10 15 20 25 30 min 1 2 3 Rs(1,2) = 3.5 12.71 4.6 x 100 mm, 3.5 mm 1 mL/min 4 N=11691 1 0 5 10 15 20 25 30 min . 2 3 N=6568 Rs(1,2) = 3.3 4.6 x 30 mm, 1.8 mm 1 mL/min 4.15 4 N=6104 0 5 10 15 20 25 30 min N=6463 4.6 x 30 mm, 1.8 mm 2 mL/min 2.09 N=6460 0 5 10 15 20 25 30 min
Ultra-fast analysis in 26 sec 4 peak width ~ 0.6 sec < 50 sec cycle time 7 analgesics separated in 21 sec for peaks 1,2,3 Total time Injection-to-Injection Higher flow rate: higher speed – same resolution – higher sensitivity/precision Sample: 7 Analgesics Column: Zorbax RRHT SB-C18, 1.8 um, 4.6 x 30 mm 2 ml/min – 90bar Rs2,1 = 2.2 Rs7,6 = 2.2 3 ml/min – 133bar 4 ml/min – 181bar Rs2,1 = 2.1 Rs7,6 = 2.2 2x faster than 2ml/min without loss in resolution! Speed
HPLC, 40°C SB-C18 4.6 x 150mm, 5µm 1.20ml/min, 40°C Analysis Time = 11min min 0 2 4 6 8 10 RRHT SB-C18 2.1mm x 50mm 1.8µm 1.00ml/min, 40°C Analysis Time= 1.1min RRLC, 40°C 10x faster 0.2 0.4 0.6 0.8 1 min 0 RRHT SB-C18 2.1mm x 50mm 1.8µm 2.40ml/min, 95°C Analysis Time: 0.4min RRLC, 95°C 27x faster 0.2 0.4 min 0.6 0 Rapid Resolution HT Columns – Up to 20X Faster • Up to 20x faster than conventional HPLC • With same or better performance (resolution, precision, sensitivity, carry over) • Compliant with strictest regulatory performance requirements >20X faster
4 5 0 1 2 3 4 5 6 7 min NON SOLO VELOCITA’ MA ANCHE EFFICIENZA 5µm: Rs(4,5) = 3.63 Peak Width, 5s (9) = 0.103 + 50% Resolution (Theory: + 67%) + 125% Efficiency (Theory: + 177%) 1.8µm: Rs(4,5) = 5.45 Peak Width 5s(9) = 0.075 Rs = +50% Colonna SB-C18 RRHT 4.6 x150mm Massima lunghezza
