Introduction to ELISA. ELISA – short for enzyme-linked immunospecific assay A technique that measures the amount of a specific protein in a solution. The Use of Assays.
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ELISA – short for enzyme-linked immunospecific assay
A technique that measures the amount of a specific protein in a solution
The 24-well plate reveals samples with different amounts of amylase activity. Amylase breaks down starch to sugar. In the activity assay, as amylase breaks down starch, the dark color of a starch/iodine mixture becomes lighter. The lighter the color, the more active the amylase.
ELISA – use an antibody to recognize a specific protein (Ag).
An enzyme bound to the Ab causes a color change in a substrate.
The more color in an ELISA, the more Ab-enzyme complex is bound to target protein, indicating a higher [Ab] present.