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Introduction to ELISA - PowerPoint PPT Presentation


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Introduction to ELISA. ELISA – short for enzyme-linked immunospecific assay A technique that measures the amount of a specific protein in a solution. The Use of Assays.

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slide1

Introduction to ELISA

ELISA – short for enzyme-linked immunospecific assay

A technique that measures the amount of a specific protein in a solution

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The Use of Assays

  • If a substance is chosen as a substance of interest, researchers and manufacturing must be able to test for its presence, activity, and concentration. The product must be “assayed.”
  • Other assays are done for potency, toxicity, and stability.
  • Assays are performed at every step in the research, development, manufacture, and quality control of a product.
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The 24-well plate reveals samples with different amounts of amylase activity. Amylase breaks down starch to sugar. In the activity assay, as amylase breaks down starch, the dark color of a starch/iodine mixture becomes lighter. The lighter the color, the more active the amylase.

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ELISA

ELISA – use an antibody to recognize a specific protein (Ag).

An enzyme bound to the Ab causes a color change in a substrate.

The more color in an ELISA, the more Ab-enzyme complex is bound to target protein, indicating a higher [Ab] present.