Solar UV Radiation. UVC <290 nm Absorbed by ozone UVB 290 – 320 nm ~0.5 % total energy UVA 320 – 400 nm 6% total energy. UV Spectrum of DNA. Absorbance. 300. 250. Wavelength (nm). T<>T. T<>C. C<>C. Pyrimidine Photodimers. Bipyrimidine (6-4) Photoadducts.
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32P-DNA + PS
Upturned Eppendorf lid
1SP + UVB
2 & 3Quenched by sodium azide & DABCO
4 & 5Mannitol & SOD have no effect
D2OSinglet oxygen damage in ss DNA increases in D2O
Specificity for 5’-G of 5’-GG-3’ doublets
5’-G of 5’-GA-3’ doublets