1 / 6

Glut

a. b. c. #55. #99. d. e. f. #221. #238. Glut. SO 4 2-. SO 4 2-. Glut. Glut. b. e. a. d. f. c. #162. #270. 31nM Ca 2+ in Glut; 174nM Ca 2+ in SO 4 2-. 012204a. 012204a. SO 4 2-. EGTA. 110904d. 50 μ M TBQ. SO 4 2- , 100 nM Ca 2+ , 0.4 mM Mg 2+.

aradia
Download Presentation

Glut

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript


  1. a b c #55 #99 d e f #221 #238 Glut SO42- SO42- Glut Glut b e a d f c #162 #270 31nM Ca2+ in Glut; 174nM Ca2+ in SO42- 012204a

  2. 012204a

  3. SO42- EGTA 110904d 50 μM TBQ SO42-, 100 nM Ca2+, 0.4 mM Mg2+ 50 mM EGTA, 1 mM Mg2+ 111804b

  4. Glut SO42- Glut 033004a

  5. Table 1: Sparks parameters from expts on Leica.

  6. A 0 s 2 min 5 s 3 min 5 s 4 min 30 s 11 min 30 s B 15 min 22 s 17 min 22 s 22 min 22 s 12 min 53 s 13 min 56 s C 23 min 53 s 24 min 54 s 26 min 23 s 28 min 23 s D Glut Glut 50 Pi Cell region Whole SR [Ca2+] (μM) Edge Middle Fig x: The effect of inorganic phosphate on SR [Ca2+]. Organelle-trapped mag-indo showing the change in SR [Ca2+] in the presence of cytoplasmic solutions containing K-glutamate, 1 mM EGTA (200 nM Ca2+) and 0.4 mM Mg2+ (A), 50 mM Pi, 1 mM EGTA (200 nM Ca2+) and 1 mM Mg2+(B) and then upon return to K-glutamate (C). The time passed since the start of the experiment is noted on each panel. The image at time 0 was in the presence of 0 Ca, 0 ATP (“relaxing”) solution. The SR [Ca2+] changes are summarized in D. Note that there were significant spatial SR [Ca2+] gradients between the edge and middle of the cell in C (indicated by the blue and green bars, respectively), which are plotted in D. The whole cell SR [Ca2+] average is plotted in red in D. Fiber identifier: 011604a.

More Related